Cy7 tyramide

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
The cells were fixed in a 1x PBS solution containing 3.7% paraformaldehyde at room temperature for 20 minutes, and then washed twice with 1x PBS.
2) Incubate in 0.1% Triton X-100 at room temperature for 1 to 5 minutes, then wash twice with 1x PBS.
3) Incubate in a 1x PBS solution containing 3% H₂O₂ for 10 minutes to block endogenous peroxidase activity, and then wash twice with 1x PBS.
4) Incubate at 4°C for 30 minutes in a 1x PBS solution containing 1% BSA.
5) Add the primary antibody working solution, incubate at room temperature for 1 hour or at 4°C overnight, and then rinse three times with 1x PBS, each time for 5 minutes.
6) Add 100 μL of the secondary antibody-HRP working solution, incubate at room temperature for 1 hour, then wash 3 times with 1x PBS, each time for 5 minutes.
7) Add 100 μL of Cy7 tyramide working solution, incubate at room temperature for 5-10 minutes, then rinse 3 times with 1x PBS, each rinse for 5 minutes.
8) Fluorescence microscopy detection.
Note: It is recommended to determine the optimal concentration of Cy7 tyramide working solution based on the specific circumstances.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.