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Cysteine thiol probe 

Cat. No.: HY-135235 Purity: 98.56%
Handling Instructions

Cysteine Thiol Probe is a thiol-based probe designed to label electrophilic natural products. Cysteine Thiol Probe possesses each of the characteristics of an ideal pharmacophore probe, and has a chromophore. Cysteine Thiol Probe is capable of engaging enone-, β-lactam-, and β-lactone-based electrophilic metabolites.

For research use only. We do not sell to patients.

Cysteine thiol probe Chemical Structure

Cysteine thiol probe Chemical Structure

CAS No. : 1947408-74-3

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 99 In-stock
Estimated Time of Arrival: December 31
5 mg USD 90 In-stock
Estimated Time of Arrival: December 31
10 mg USD 150 In-stock
Estimated Time of Arrival: December 31
50 mg USD 450 In-stock
Estimated Time of Arrival: December 31
100 mg USD 750 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Cysteine Thiol Probe is a thiol-based probe designed to label electrophilic natural products. Cysteine Thiol Probe possesses each of the characteristics of an ideal pharmacophore probe, and has a chromophore. Cysteine Thiol Probe is capable of engaging enone-, β-lactam-, and β-lactone-based electrophilic metabolites[1][2].

In Vitro

Cysteine Thiol Probe (Probe 1) reacts much more readily with β-lactam- and β-lactone-based electrophilic natural products, and its reactivity with epoxide-based electrophilic natural products is poor. And probe 2 reacts much faster with epoxide-containing natural products. This orthogonal reactivity allows them to be employed in extracts simultaneously. Since Cysteine Thiol Probe is brominated and probe 2 is chlorinated, the isotopic patterns of labeled natural products point toward their structural origin. Competition experiments using Cysteine Thiol Probe and 2 indicated that brominated Cysteine Thiol Probe reacted exclusively with the β-lactam in penicillin G and the β-lactone in salinosporamide A while chlorinated probe 2 alone reacted with the epoxide in salinamide A[1].

Molecular Weight

318.19

Formula

C₁₁H₁₂BrNO₃S

CAS No.

1947408-74-3

SMILES

SC[[email protected]@H](C(OC)=O)NC(C1=CC=C(Br)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (785.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1428 mL 15.7139 mL 31.4278 mL
5 mM 0.6286 mL 3.1428 mL 6.2856 mL
10 mM 0.3143 mL 1.5714 mL 3.1428 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.54 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.54 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

Cysteine thiol probeOthersNaturalproductsl-cystinepharmacophorechromophoreelectrophilicenone-basedβ-lactam-basedβ-lactone-basedthiol-basedInhibitorinhibitorinhibit

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Cysteine thiol probe
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HY-135235
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