D-DT/MIF-1-IN-1
D-DT/MIF-1-IN-1 (Compound 4h) is a non-competitive, non-covalent inhibitor of MIF-1 and D-DT, with IC50 values of 2.4 μM and 4.0 μM against D-DT, and an IC50 value of 9.8 μM against MIF-1. D-DT/MIF-1-IN-1 inhibits D-DT-induced phosphorylation of ERK and exerts antiproliferative activity in non-small cell lung cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 1461756-61-5
- Formula: C12H10ClNO3
- Molecular Weight:251.67
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
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MIF-1 9.8 μM (IC50) |
D-DT/MIF-1-IN-1 (compound 4h) (tested to determine IC50 values; 10 min preincubation at room temperature) potently inhibits recombinant human D-DT tautomerase activity with an IC50 of 2.4 ± 0.6 μM and moderately inhibits recombinant human MIF-1 tautomerase activity with an IC50 of 9.8 ± 0.5 μM[1].
D-DT/MIF-1-IN-1 (compound 4h) (1.6-100 μM; 72 h) exhibits dose-dependent antiproliferative activity against H1299 NSCLC cells with an IC50 of ~25 μM, and only inhibits A549 NSCLC cell proliferation at high concentrations[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 NSCLC cell line
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Concentration:10, 20 μM
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Incubation Time:10 min
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Result:Attenuated D-DT-induced ERK phosphorylation in a dose-dependent manner, reducing pERK levels compared to D-DT-only stimulated cells.
Chemical Information
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CAS No. 1461756-61-5
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Molecular Weight 251.67
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Formula C12H10ClNO3
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SMILES
OC(C1=CC=C(O1)NCC2=CC=C(C=C2)Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)