2. Immunology/Inflammation Stem Cell/Wnt MAPK/ERK Pathway
  3. Macrophage migration inhibitory factor (MIF) ERK
  4. D-DT/MIF-1-IN-1

D-DT/MIF-1-IN-1 (Compound 4h) is a non-competitive, non-covalent inhibitor of MIF-1 and D-DT, with IC50 values of 2.4 μM and 4.0 μM against D-DT, and an IC50 value of 9.8 μM against MIF-1. D-DT/MIF-1-IN-1 inhibits D-DT-induced phosphorylation of ERK and exerts antiproliferative activity in non-small cell lung cancer cells.

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D-DT/MIF-1-IN-1

D-DT/MIF-1-IN-1 Chemical Structure

CAS No. : 1461756-61-5

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D-DT/MIF-1-IN-1 Related Antibodies

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Description

D-DT/MIF-1-IN-1 (Compound 4h) is a non-competitive, non-covalent inhibitor of MIF-1 and D-DT, with IC50 values of 2.4 μM and 4.0 μM against D-DT, and an IC50 value of 9.8 μM against MIF-1. D-DT/MIF-1-IN-1 inhibits D-DT-induced phosphorylation of ERK and exerts antiproliferative activity in non-small cell lung cancer cells[1].

IC50 & Target[1]

MIF-1

9.8 μM (IC50)

In Vitro

D-DT/MIF-1-IN-1 (compound 4h) (tested to determine IC50 values; 10 min preincubation at room temperature) potently inhibits recombinant human D-DT tautomerase activity with an IC50 of 2.4 ± 0.6 μM and moderately inhibits recombinant human MIF-1 tautomerase activity with an IC50 of 9.8 ± 0.5 μM[1].
D-DT/MIF-1-IN-1 (compound 4h) (1.6-100 μM; 72 h) exhibits dose-dependent antiproliferative activity against H1299 NSCLC cells with an IC50 of ~25 μM, and only inhibits A549 NSCLC cell proliferation at high concentrations[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D-DT/MIF-1-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 NSCLC cell line
Concentration: 10, 20 μM
Incubation Time: 10 min
Result: Attenuated D-DT-induced ERK phosphorylation in a dose-dependent manner, reducing pERK levels compared to D-DT-only stimulated cells.
Molecular Weight

251.67

Formula

C12H10ClNO3
CAS No.
SMILES

OC(C1=CC=C(O1)NCC2=CC=C(C=C2)Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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D-DT/MIF-1-IN-1 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-DT/MIF-1-IN-11461756-61-5Macrophage migration inhibitory factor (MIF)ERKExtracellular signal regulated kinasestautomerase activityERK signalingH1299 NSCLC cellsA549 NSCLC cellsnon-small cell lung cancer cellsERK phosphorylationD-dopachrome tautomeraseMacrophage Migration Inhibitory Factor-1Inhibitorinhibitorinhibit

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