RB-OPD
Based on 1 Customer Validation
RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm).
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 1034863-51-8
- Formula: C34H36N4O2
- Molecular Weight:532.68
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
RB-OPD (compound 1) exhibits "turn-on" type fluorogenic and chromogenic behavior toward NO in aqueous solution with great sensitivity and selectivity, and it can be used for the sensing of NO under normal physiological conditions. NO-red is composed of two moieties: rhodamine B spirolactam as the potential strong fluorophore and chromophore, and o-phenylenediamine as an NO-reactive group in a lactam form as a "masked" NO-sensitive modulator[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
590
550
Chemical Information
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CAS No. 1034863-51-8
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Appearance Solid
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Molecular Weight 532.68
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Formula C34H36N4O2
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Color Off-white to light yellow
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SMILES
O=C1C2=C(C3(N1C4=C(N)C=CC=C4)C5=CC=C(N(CC)CC)C=C5OC6=CC(N(CC)CC)=CC=C63)C=CC=C2
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Synonyms
NO-red
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (46.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8773 mL | 9.3865 mL | 18.7730 mL | 46.9325 mL |
| 5 mM | 0.3755 mL | 1.8773 mL | 3.7546 mL | 9.3865 mL | |
| 10 mM | 0.1877 mL | 0.9386 mL | 1.8773 mL | 4.6932 mL | |
| 15 mM | 0.1252 mL | 0.6258 mL | 1.2515 mL | 3.1288 mL | |
| 20 mM | 0.0939 mL | 0.4693 mL | 0.9386 mL | 2.3466 mL | |
| 25 mM | 0.0751 mL | 0.3755 mL | 0.7509 mL | 1.8773 mL | |
| 30 mM | 0.0626 mL | 0.3129 mL | 0.6258 mL | 1.5644 mL | |
| 40 mM | 0.0469 mL | 0.2347 mL | 0.4693 mL | 1.1733 mL |