Di-12-ANEPPQ
Di-12-ANEPPQ is a fast-responding membrane potential dye. Di-12-ANEPPQ, the lipophilic dye, shows cell-specific loading and Golgi-like staining patterns with minimal background fluorescence in the slices of neocortex and hippocampus.
For research use only. We do not sell to patients.
- CAS No.: 217176-86-8
- Formula: C47H77Br2N3
- Molecular Weight:843.94
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Emission (Em)
720
Excitation (Ex)
522
Chemical Information
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CAS No. 217176-86-8
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Molecular Weight 843.94
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Formula C47H77Br2N3
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SMILES
CCCCCCCCCCCCN(CCCCCCCCCCCC)C1=CC=C2C(C=CC(/C=C/C3=CC=[N+](CCC[N+](C)(C)C)C=C3)=C2)=C1.[Br-].[Br-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)