diABZI-V/C-DBCO
Based on 1 Customer Validation
diABZI-V/C-DBCO is a STING agonist with an EC50 of 1.47 nM. diABZI-V/C-DBCO activates the STING pathway, induces the production of IFN-I, and stimulates the secretion of IFN-β. diABZI-V/C-DBCO serves as a substrate for cathepsin B, and releases active diABZI-amine via cathepsin B-mediated cleavage. In an orthotopic mouse model of breast cancer, diABZI-V/C-DBCO increases serum IFN-β levels and the frequency of granzyme B+ CD8+ T cells. diABZI-V/C-DBCO is applicable to research related to triple-negative breast cancer.
For research use only. We do not sell to patients.
- Purity: 98.66%
- CAS No.: 3035557-60-6
- Formula: C78H89N19O13
- Molecular Weight:1500.66
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
diABZI-V/C-DBCO (0.000001-100 μM, 24 h) activates the STING pathway in THP1-Dual cells with an EC50 of 1.47 nM, and induces the production of type I interferon (IFN-I)[1].
diABZI-V/C-DBCO (0.001-100 μM, 24 h) stimulates mouse splenocytes to secrete beta interferon (IFN-β), with an EC50 of 7.7 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:mouse splenocytes
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Concentration:0.001, 0.01, 1, 10, 100 μM
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Incubation Time:24 h
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Result:Stimulated mouse splenocytes to secrete beta interferon (IFN-β), with an EC50 of 7.7 μM
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (female, 7 weeks old, EO771 breast cancer cell inoculation model)[1]
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Dosage:0.009 μmol/mouse (0.68 mg/kg)
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Administration:i.v.; single dose/3 doses; once every 4 days
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Result:Failed to significantly upregulate STING-related genes (Ifnb1, Cxcl10, Tmem173, Tnf, Il6) in tumor tissue relative to PBS control.
Increased serum IFN-β levels relative to PBS but lower than levels induced by SAPCon[100 kDa].
Increased the frequency of granzyme B+ CD8+ T cells relative to PBS.
Chemical Information
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CAS No. 3035557-60-6
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Appearance Solid
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Molecular Weight 1500.66
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Formula C78H89N19O13
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Color White to off-white
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SMILES
CCN1N=C(C=C1C(NC2=NC3=C(N2C/C=C/CN4C(NC(C5=CC(C)=NN5CC)=O)=NC6=CC(C(N)=O)=CC(OC)=C64)C(OCCCNC(OCC7=CC=C(C=C7)NC([C@@H](NC([C@H](C(C)C)NC(CCCCC(N8CC9=C(C=CC=C9)C#CC%10=C8C=CC=C%10)=O)=O)=O)CCCNC(N)=O)=O)=O)=CC(C(N)=O)=C3)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (66.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.6664 mL | 3.3319 mL | 6.6637 mL | 16.6593 mL |
| 5 mM | 0.1333 mL | 0.6664 mL | 1.3327 mL | 3.3319 mL | |
| 10 mM | 0.0666 mL | 0.3332 mL | 0.6664 mL | 1.6659 mL | |
| 15 mM | 0.0444 mL | 0.2221 mL | 0.4442 mL | 1.1106 mL | |
| 20 mM | 0.0333 mL | 0.1666 mL | 0.3332 mL | 0.8330 mL | |
| 25 mM | 0.0267 mL | 0.1333 mL | 0.2665 mL | 0.6664 mL | |
| 30 mM | 0.0222 mL | 0.1111 mL | 0.2221 mL | 0.5553 mL | |
| 40 mM | 0.0167 mL | 0.0833 mL | 0.1666 mL | 0.4165 mL | |
| 50 mM | 0.0133 mL | 0.0666 mL | 0.1333 mL | 0.3332 mL | |
| 60 mM | 0.0111 mL | 0.0555 mL | 0.1111 mL | 0.2777 mL |
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