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  5. DMBA

DMBA  (Synonyms: 7,12-ジメチルベンズ[a]アントラセン)

製品番号: HY-W011845 純度: 99.94%
COA 取扱説明書 Technical Support

DMBA has carcinogenic activity as a polycyclic aromatic hydrocarbon (PAH). DMBA is used to induce tumor formation in various rodent models.

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DMBA

DMBA 構造式

CAS 番号 : 57-97-6

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 38 在庫あり
Solution
10 mM * 1 mL in DMSO USD 38 在庫あり
Solid
25 mg $35 在庫あり
50 mg $50 在庫あり
100 mg $80 在庫あり
250 mg $160 在庫あり
1 g $410 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 3 publication(s) in Google Scholar

Other Forms of DMBA:

DMBA 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

DMBA has carcinogenic activity as a polycyclic aromatic hydrocarbon (PAH). DMBA is used to induce tumor formation in various rodent models[1].

体内実験

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

DMBA can induce breast cancer and skin cancer models. Oral administration of DMBA (0-150 mg/kg) leads to a reduction in spleen weight and a decrease in the total number of lymphocytes recovered from the spleen in C57BL/6 mice. In SD rats, DMBA exhibits a terminal deposition half-life of 45 minutes across all dose ranges (5-50 mg)[5].

Induction of breast cancer[2]
Background
DMBA activates AhR, which translocates into nucleus and dimerizes with cofactor ARNT, thus induces the expressions of protooncogenes c-myc.
Specific Modeling Methods
Rat: Sprague-Dawley • female • 50 days •140-150 g
Administration: 15-20 mg DMBA-• intragastric gavage • single dose
Note
1.5-2 g DMBA dissolved in 100 ml sesame oil
Modeling Indicators
Palpation:Detection of mammary cancer in 150 days.
Histology analysis:The tumor exhibits tightly spaced chords epithelial cells, cysts and papillary structures.
Phenotype:develops a temporary cessation in body growth.
Opposite Product(s): Phytic acid (HY-N0814); Limonene (HY-N0544)

Induction of skin cancer[3]
Background
DMBA is metabolized to reactive intermediates, such as syn- and anti-diol epoxides, and binds extensively to epidermal DNA.
Specific Modeling Methods
European hamster: • 10 weeks • 180-200 g •
Administration: 25 mg DMBA • applied on the shaved back skin • single dose
Note
25 mg DMBA is dissolved in 1 ml acetone
Modeling Indicators
Histological analysis: Epidermal hyperplasia is oberserved. Lesions occurred frequently in the back skin, such as trichoepitheliomas, neoplastic epidermal cysts and melanotic tumors.
Opposite Product(s): α-Mangostin (HY-N0328); DHEA (HY-14650); Salidroside (HY-N0109)

Induction of lung cancer[4]
Background
DMBA is metabolized by cytochrome P450 enzymes into the carcinogenic form 7,12-DMBA-3,4-dihydrodiol-1,2- epoxide, which adducts with DNA and produces mutations.
Specific Modeling Methods
BALB/c mice: •12 weeks • 25-35 g
Administration: 5-20 mg/kg • intratracheal instillation • single dose.
Note
(1). 5-20 mg DMBA is dissolved in 0.2 ml saline.
(2). A coat of antibiotic is applied after the instillation.
Modeling Indicators
Molecular changes:Increased number of macrophages in the broncho-alveolar lavage (BAL) fluid and levels of malonyldialdehyde (MDA) in lung tissue.
Histology analysis:Inflammation with alveolar epithelial hyperplasia, adenic metaplasia, adenomatoid hyperplasia and atypical hyperplasia.
Opposite Product(s): Diclofenac (HY-15036)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

256.35

分子式

C20H16
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

CC(C1=CC=CC=C21)=C3C=CC4=CC=CC=C4C3=C2C
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

Acetone : 25 mg/mL (97.52 mM; Need ultrasonic)

DMSO : ≥ 12.5 mg/mL (48.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 3.33 mg/mL (12.99 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9009 mL 19.5046 mL 39.0092 mL
5 mM 0.7802 mL 3.9009 mL 7.8018 mL
10 mM 0.3901 mL 1.9505 mL 3.9009 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (9.75 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2 mg/mL (7.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  Corn Oil

    Solubility: 20 mg/mL (78.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.94%

COA (251 KB) HNMR (245 KB) RP-HPLC (255 KB) MS (72 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO / Acetone 1 mM 3.9009 mL 19.5046 mL 39.0092 mL 97.5229 mL
5 mM 0.7802 mL 3.9009 mL 7.8018 mL 19.5046 mL
10 mM 0.3901 mL 1.9505 mL 3.9009 mL 9.7523 mL
DMSO / Acetone 15 mM 0.2601 mL 1.3003 mL 2.6006 mL 6.5015 mL
20 mM 0.1950 mL 0.9752 mL 1.9505 mL 4.8761 mL
25 mM 0.1560 mL 0.7802 mL 1.5604 mL 3.9009 mL
30 mM 0.1300 mL 0.6502 mL 1.3003 mL 3.2508 mL
40 mM 0.0975 mL 0.4876 mL 0.9752 mL 2.4381 mL
Acetone 50 mM 0.0780 mL 0.3901 mL 0.7802 mL 1.9505 mL
60 mM 0.0650 mL 0.3251 mL 0.6502 mL 1.6254 mL
80 mM 0.0488 mL 0.2438 mL 0.4876 mL 1.2190 mL
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DMBA Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMBA57-97-67,12-DMBA 7,12-DimethylbenzanthraceneEnvironmental Pollutantsc-MycEnvironmental ContaminantsMycInhibitorinhibitorinhibit

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