Dornase alfa
Based on 1 publication(s) in Google Scholar
Dornase alfa (rhDNase) is a recombinant human deoxyribonuclease I (rhDNase) that can specifically degrade extracellular DNA. Dornase alfa catalyzes the cleavage of DNA released by neutrophils in respiratory mucus, reduces sputum viscosity, thereby improving mucus clearance efficiency, reducing airway obstruction and alleviating inflammatory responses. Dornase alfa can be used to improve lung function (such as FEV_1) in cystic fibrosis (CF), reduce the risk of acute pulmonary exacerbations, and has good in vivo tolerability. Dornase alfa acts locally on the respiratory tract through aerosol inhalation, specifically improving the high viscosity of mucus caused by DNA accumulation and related respiratory symptoms.
For research use only. We do not sell to patients.
- CAS No.: 143831-71-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Dornase alfa
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Biological Activity
In vitro enzyme activity assays of Dornase alfa (2.5 mg/2.5 mL; nebulized) showed that after treatment with different types of nebulizers (spray type, static type, and vibration type), there was no significant loss of enzyme activity, and the protein structure remained intact without aggregation or degradation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Dornase alfa can reduce the number of tumor metastases in a mouse B16 melanoma model and change the cfDNA pattern in serum[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c mice (7-8 weeks old), healthy murine model[3]
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Dosage:2.5 mg/2.5 mL
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Administration:Nebulization via four nebulizers (jet nebulizers: PARI BOY SX-red/blue nozzles; static mesh: NE-U150; vibrating mesh: NE-SM1), single administration for 1 minute after 30 seconds of pre-nebulization
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Result:The PARI BOY SX-red nozzle jet nebulizer delivered the highest amount of dornase alfa to the lungs, correlating with its aerosol particle size (Dv(50)=2.5-3.0 μm).
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
1000 IU/mg protein
One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5.
Chemical Information
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CAS No. 143831-71-4
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Dornase alfa]
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Synonyms
rhDNase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Carbohydr Polym
Exploring the antibiofilm potential of chitosan nanoparticles by functional modification with chloroquine and deoxyribonuclease. [Abstract]2025 Jan 1:347:122726. PMID: 39486956
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Connie Yang, et al. Dornase alfa for cystic fibrosis. Cochrane Database Syst Rev. 2021 Mar 18;3(3):CD001127. [Content Brief]
[2]. Konstan MW, et al. Effect of dornase alfa on inflammation and lung function: potential role in the early treatment of cystic fibrosis. J Cyst Fibros. 2012 Mar;11(2):78-83. [Content Brief]
[3]. Chang KH, et al. Aerosol Delivery of Dornase Alfa Generated by Jet and Mesh Nebulizers. Pharmaceutics. 2020 Jul 31;12(8):721. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dornase alfa Related Classifications
HY-108858 Related Classifications
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