DOTA-NT-20.3-IPBA

DOTA-NT-20.3-IPBA binds to human albumin (PDB ID: 5yoq) with a binding energy of −8.1 kcal/mol, forming hydrogen bonds with 10 specific amino acid residues^[1].
DOTA-NT-20.3-IPBA binds to human NTR1 (PDB ID: 4grv) with a binding energy of −8.7 kcal/mol, forming hydrogen bonds with 9 specific amino acid residues^[1].
DOTA-NT-20.3-IPBA (20 μg; 10 min heated at 95 °C) can be radiolabeled with ⁶⁸Ga to produce [⁶⁸Ga]Ga-DOTA-NT-20.3-IPBA with >95% radiochemical yield, >95% radiochemical purity, and a molar activity of 5−6 MBq/nmol^[1].
DOTA-NT-20.3-IPBA (~0.74 MBq) has a LogP value of -2.37 following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~0.37 MBq; 1 h) binds to mouse serum albumin at a rate of 19.27% following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~1.48 MBq; 1-2 h) maintains >95% intactness following labeling with ⁶⁸Ga after 2 h of in vitro incubation in PBS at room temperature or mouse serum at 37 °C^[1].
DOTA-NT-20.3-IPBA (5-100 nM; 1 h) binds to NTR1 on HT-29 cells with a dissociation constant (K_d) of 33.86 nM following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~0.15 MBq; 1-3 h) is taken up at significantly higher levels by NTSR1-high expressing HT-29 cells than NTSR1-low expressing SKOV3 cells following labeling with ⁶⁸Ga, and this uptake is specifically inhibited by excess NT-20.3, demonstrating NTR1-specific binding^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.