DOTA-NT-20.3-IPBA 

DOTA-NT-20.3-IPBA is a Neurotensin receptor 1 (NTR1)-targeting albumin binder with specific high-affinity binding to NTR1. DOTA-NT-20.3-IPBA is formed by the conjugation of DOTA-NT-20.3 and IPBA. DOTA-NT-20.3-IPBA can be used for positron emission tomography (PET) imaging following labeling with [⁶⁸Ga]Ga^[1].

DOTA-NT-20.3-IPBA binds to human albumin (PDB ID: 5yoq) with a binding energy of −8.1 kcal/mol, forming hydrogen bonds with 10 specific amino acid residues^[1].
DOTA-NT-20.3-IPBA binds to human NTR1 (PDB ID: 4grv) with a binding energy of −8.7 kcal/mol, forming hydrogen bonds with 9 specific amino acid residues^[1].
DOTA-NT-20.3-IPBA (20 μg; 10 min heated at 95 °C) can be radiolabeled with ⁶⁸Ga to produce [⁶⁸Ga]Ga-DOTA-NT-20.3-IPBA with >95% radiochemical yield, >95% radiochemical purity, and a molar activity of 5−6 MBq/nmol^[1].
DOTA-NT-20.3-IPBA (~0.74 MBq) has a LogP value of -2.37 following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~0.37 MBq; 1 h) binds to mouse serum albumin at a rate of 19.27% following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~1.48 MBq; 1-2 h) maintains >95% intactness following labeling with ⁶⁸Ga after 2 h of in vitro incubation in PBS at room temperature or mouse serum at 37 °C^[1].
DOTA-NT-20.3-IPBA (5-100 nM; 1 h) binds to NTR1 on HT-29 cells with a dissociation constant (K_d) of 33.86 nM following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~0.15 MBq; 1-3 h) is taken up at significantly higher levels by NTSR1-high expressing HT-29 cells than NTSR1-low expressing SKOV3 cells following labeling with ⁶⁸Ga, and this uptake is specifically inhibited by excess NT-20.3, demonstrating NTR1-specific binding^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DOTA-NT-20.3-IPBA (~3.7 MBq; i.v.; single injection) achieves specific, sustained NTR1-targeted tumor uptake in HT-29 xenograft mice following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (7.4 MBq; i.v.; single injection) exhibits a prolonged systemic circulation half-life of 30.43 minutes in normal mice following labeling with ⁶⁸Ga^[1].
DOTA-NT-20.3-IPBA (~7.4 MBq; i.v.; single injection) causes no detectable organ damage in healthy BALB/c nude mice at 24 hours postinjection following labeling with ⁶⁸Ga^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1712.77

C₇₆H₁₁₈IN₁₉O₁₈

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhu J, et al. A Novel Radiotracer for NTSR1-Targeted PET Imaging of Tumor: Preclinical and First-in-Human Studies. J Med Chem. 2025;68(23):25186-25197.  [Content Brief]