Bacteriological Dyes

sBADA TFA is a potent green fluorescent dye that targets and labels peptidoglycan in the cell walls of live bacteria(Ex/Em = 490/510 nm).

sBADA is a potent green fluorescent dye that targets and labels peptidoglycan in the cell walls of live bacteria(Ex/Em = 490/510 nm).

Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm).

Phenosafranine is a phenazine dye. Phenosafranine exhibits higher binding affinity for triple-stranded RNA than for double-stranded RNA, and binds to both types of RNA via intercalation. Phenosafranine interacts with hemoglobin. Phenosafranine can be used for plant cell staining, as well as the detection of hemoglobin, dopamine, serotonin, etc.

Benzenepentacarboxylic acid is a fluorescent dye that detects and scavenge HO radicals.

sCy5DL-amide is an amidated fluorescent D-amino acid conjugated to Sulfo-Cyanine 5, and a peptidoglycan labeling agent. sCy5DL-amide incorporates into bacterial peptidoglycan cell wall via transpeptidase-mediated reactions, replacing the fourth or fifth D-amino acid of the peptidoglycan stem peptide for visualization of nascent peptidoglycan biosynthesis. sCy5DL-amide produces clear cell outline, septum labeling, and high localization density in Bacillus subtilis, including a 'V-shape' pattern at cell-cell contact areas. sCy5DL-amide exhibits robust incorporation into Gram-positive bacteria and mycobacteria, with reduced incorporation into Gram-negative bacteria (Ex/Em = 646/666 nm).

RADA is a fluorescent D-amino acid (FDAA) with high photostability and thermostability, which emits yellow-to-orange fluorescence. RADA shows low outer membrane permeability in wild-type Gram-negative Escherichia coli, but it targets penicillin-binding proteins and L,D-transpeptidases, mimics the interaction between acyl acceptors and enzyme intermediates, and integrates into peptidoglycan during biosynthesis. As a peptidoglycan labeling reagent, RADA metabolically integrates into the nascent peptidoglycan of live bacterial cells, labels the peptidoglycan at the poles and lateral walls of mycobacteria, and enables visualization of peptidoglycan synthesis and remodeling processes. RADA serves as a non-specific stain for fixed cells, is non-toxic to bacterial cells, and its red-shifted excitation/emission spectra reduce phototoxicity. RADA also supports virtual pulse-chase labeling experiments and stochastic optical reconstruction microscopy for sub-diffraction-limited imaging of bacterial cell walls.

RMR-Tre is a fluorescent probe targeting the mycobacterial acyltransferase Ag85. Under the catalysis of Ag85, RMR-Tre undergoes 6-position mycoloylation and anchors to the mycobacterial membrane, while achieving fluorescence activation by inhibiting the intramolecular twisted charge transfer state transition. RMR-Tre can distinguish live mycobacteria from dead ones through metabolism-driven labeling, enabling rapid, wash-free, low-background detection of viable bacteria. RMR-Tre reports the drug resistance of Mycobacterium tuberculosis via the trehalose catalytic shift activity readout associated with TreS. In addition, RMR-Tre can be combined with flow cytometry or high-content imaging techniques to visualize and quantitatively analyze the metabolic heterogeneity of Mycobacterium tuberculosis related to persistence and drug resistance. RMR-Tre is widely used in tuberculosis-related research.

Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
Solvent and concentration: PBS: 5 mg/mL

CDG-DNB3 is a selective fluorescent probe for Mycobacterium tuberculosis. CDG-DNB3 provides rapid and specific labeling of live Mycobacterium tuberculosis. CDG-DNB3 can image Bacillus Calmette-Guérin phagocytosis in real time.

BacGO is a highly selective, wash free fluorescent probe for Gram positive bacteria. BacGO binds to the carbohydrate structure in peptidoglycan through boric acid and exhibits depolymerization induced luminescence (DIE) properties. BacGO can be used for imaging complex environmental samples (such as activated sludge) and flat plate bacteria without affecting bacterial activity.

N14G-Fe, the Fe³⁺-chelated form of N14G, identifies Mtb in sputum samples with tuberculosis, exhibiting exceptional fluorescence. N14G-Fe can effectively traverse the cell wall and inner membrane region where IrtAB is located.

OGDA is a green fluorescent D-amino acid. OGDA is suitable for labeling peptidoglycan in Gram-positive and Gram-negative bacteria.

1,3-Di-(2-pyrenyl)propane is a fluorescent indicator. 1,3-Di-(2-pyrenyl)propane can be used in fluidity of bacterial membrane lipids monitored by intramolecular excimerization. 1,3-Di-(2-pyrenyl)propane shows temperature dependence of fluorescence lifetime distributions.

Acid Red 94 sodium (80%), a synthetic fluorescein derivative, is a deep red dye primarily composed of 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo fluorescein. Acid Red 94 sodium (80%) is widely used as an ophthalmic diagnostic agent to detect dry or damaged cells on the ocular surface. Acid Red 94 sodium (80%) exhibits antitumor activity and can inhibit Friend Leukemia Virus (FLV) infection through photodynamic action. Additionally, Acid Red 94 sodium (80%) can inhibit Aβ aggregation through light irradiation. Acid Red 94 sodium (80%) holds potential for use in cancer, viral infections, and neurodegenerative disease research.

Cy3-NO2-Tre is a nitroreductase-responsive cyanine-based fluorescent probe that specifically labels Mycobacterium tuberculosis (Mtb). Cy3-NO2-tre generated fluorescence after activation by a specific nitroreductase, Rv3368c, which is conserved in the Mycobacteriaceae. Cy3-NO2-tre effectively imaged mycobacteria within infected host cells, tracked the infection process, and visualized Mycobacterium smegmatis being endocytosed by macrophages (Ex/Em= 540-550/575-625 nm).

N14G is a fluorescent dye that can be used to stain bacteria such as Mycobacterium tuberculosis.

NFC nitro probe 1 (compound 18) is an NFC termed probe for Mtb detection. NFC nitro probe 1 (compound 18) shows high potency for R-Mtb and NR-Mtb.

Cy3-NO2 is a cyanine-based probe. Cy3-NO2 can be excited by using a 554 nm laser and filtered with a 568 nm laser.