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  4. Phenosafranine

Phenosafranine is a phenazine dye. Phenosafranine exhibits higher binding affinity for triple-stranded RNA than for double-stranded RNA, and binds to both types of RNA via intercalation. Phenosafranine interacts with hemoglobin. Phenosafranine can be used for plant cell staining, as well as the detection of hemoglobin, dopamine, serotonin, etc.

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Phenosafranine

Phenosafranine Chemical Structure

CAS No. : 81-93-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

Phenosafranine Related Antibodies

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Description

Phenosafranine is a phenazine dye. Phenosafranine exhibits higher binding affinity for triple-stranded RNA than for double-stranded RNA, and binds to both types of RNA via intercalation. Phenosafranine interacts with hemoglobin. Phenosafranine can be used for plant cell staining, as well as the detection of hemoglobin, dopamine, serotonin, etc.[1][2][3]

In Vitro

Phenosafranine (0.1%; 5-30 min) serves as a specific and rapid stain for dead plant cells in suspension culture, with applicability to Nicotiana tabacum cv. Xanthi, Nicotiana tabacum cv. Wisconsin 38, Oryza sativa cv. Blue Bonnett 50, Glycine max cv. Wayne, carrot, and tomato[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phenosafranine Related Antibodies
Molecular Weight

322.80

Formula

C18H15ClN4
CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

NC1=CC2=[N+](C3=C(N=C2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (387.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0979 mL 15.4895 mL 30.9789 mL
5 mM 0.6196 mL 3.0979 mL 6.1958 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 90.77%

SDS (394 KB)

COA (249 KB) HNMR (265 KB) LCMS (104 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0979 mL 15.4895 mL 30.9789 mL 77.4473 mL
5 mM 0.6196 mL 3.0979 mL 6.1958 mL 15.4895 mL
10 mM 0.3098 mL 1.5489 mL 3.0979 mL 7.7447 mL
15 mM 0.2065 mL 1.0326 mL 2.0653 mL 5.1632 mL
20 mM 0.1549 mL 0.7745 mL 1.5489 mL 3.8724 mL
25 mM 0.1239 mL 0.6196 mL 1.2392 mL 3.0979 mL
30 mM 0.1033 mL 0.5163 mL 1.0326 mL 2.5816 mL
40 mM 0.0774 mL 0.3872 mL 0.7745 mL 1.9362 mL
50 mM 0.0620 mL 0.3098 mL 0.6196 mL 1.5489 mL
60 mM 0.0516 mL 0.2582 mL 0.5163 mL 1.2908 mL
80 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9681 mL
100 mM 0.0310 mL 0.1549 mL 0.3098 mL 0.7745 mL
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Phenosafranine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenosafranine81-93-6Fluorescent Dyephenazine dyeplant cell staininghemoglobinInhibitorinhibitorinhibit

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