Phenosafranine
Based on 1 Customer Validation
Phenosafranine is a phenazine dye. Phenosafranine exhibits higher binding affinity for triple-stranded RNA than for double-stranded RNA, and binds to both types of RNA via intercalation. Phenosafranine interacts with hemoglobin. Phenosafranine can be used for plant cell staining, as well as the detection of hemoglobin, dopamine, serotonin, etc.
For research use only. We do not sell to patients.
- Purity: 90.77%
- CAS No.: 81-93-6
- Formula: C18H15ClN4
- Molecular Weight:322.80
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Phenosafranine (0.1%; 5-30 min) serves as a specific and rapid stain for dead plant cells in suspension culture, with applicability to Nicotiana tabacum cv. Xanthi, Nicotiana tabacum cv. Wisconsin 38, Oryza sativa cv. Blue Bonnett 50, Glycine max cv. Wayne, carrot, and tomato[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 81-93-6
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Appearance Solid
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Molecular Weight 322.80
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Formula C18H15ClN4
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Color Green to dark green
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SMILES
NC1=CC2=[N+](C3=C(N=C2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 125 mg/mL (387.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[2]. Widholm JM, et al. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94. [Content Brief]
[3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016;86:345-351. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0979 mL | 15.4895 mL | 30.9789 mL | 77.4473 mL |
| 5 mM | 0.6196 mL | 3.0979 mL | 6.1958 mL | 15.4895 mL | |
| 10 mM | 0.3098 mL | 1.5489 mL | 3.0979 mL | 7.7447 mL | |
| 15 mM | 0.2065 mL | 1.0326 mL | 2.0653 mL | 5.1632 mL | |
| 20 mM | 0.1549 mL | 0.7745 mL | 1.5489 mL | 3.8724 mL | |
| 25 mM | 0.1239 mL | 0.6196 mL | 1.2392 mL | 3.0979 mL | |
| 30 mM | 0.1033 mL | 0.5163 mL | 1.0326 mL | 2.5816 mL | |
| 40 mM | 0.0774 mL | 0.3872 mL | 0.7745 mL | 1.9362 mL | |
| 50 mM | 0.0620 mL | 0.3098 mL | 0.6196 mL | 1.5489 mL | |
| 60 mM | 0.0516 mL | 0.2582 mL | 0.5163 mL | 1.2908 mL | |
| 80 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9681 mL | |
| 100 mM | 0.0310 mL | 0.1549 mL | 0.3098 mL | 0.7745 mL |
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