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  3. Fluorescent Dye
  4. Phenosafranine

Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on.

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Phenosafranine Chemical Structure

Phenosafranine Chemical Structure

CAS No. : 81-93-6

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Phenosafranine Related Antibodies

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Description

Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].

Molecular Weight

322.79

Formula

C18H15ClN4
CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

NC1=CC2=[N+](C3=C(N=C2C=C1)C=CC(N)=C3)C4=CC=CC=C4.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (387.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0980 mL 15.4899 mL 30.9799 mL
5 mM 0.6196 mL 3.0980 mL 6.1960 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

V1

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0980 mL 15.4899 mL 30.9799 mL 77.4497 mL
5 mM 0.6196 mL 3.0980 mL 6.1960 mL 15.4899 mL
10 mM 0.3098 mL 1.5490 mL 3.0980 mL 7.7450 mL
15 mM 0.2065 mL 1.0327 mL 2.0653 mL 5.1633 mL
20 mM 0.1549 mL 0.7745 mL 1.5490 mL 3.8725 mL
25 mM 0.1239 mL 0.6196 mL 1.2392 mL 3.0980 mL
30 mM 0.1033 mL 0.5163 mL 1.0327 mL 2.5817 mL
40 mM 0.0774 mL 0.3872 mL 0.7745 mL 1.9362 mL
50 mM 0.0620 mL 0.3098 mL 0.6196 mL 1.5490 mL
60 mM 0.0516 mL 0.2582 mL 0.5163 mL 1.2908 mL
80 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9681 mL
100 mM 0.0310 mL 0.1549 mL 0.3098 mL 0.7745 mL
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Phenosafranine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenosafranine81-93-6Fluorescent DyephenazineRNA bindingstainingInhibitorinhibitorinhibit

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