eGFP mRNA 

eGFP mRNA expresses green fluorescent protein derived from the jellyfish Aequorea victoria. eGFP is a commonly used direct-detection reporter gene in mammalian cell culture, which produces bright green fluorescence. This product is synthesized without modified nucleosides, with a Cap1 version cap analog at the 5' end and a 100nt PolyA tail at the 3' end. It can be used as a positive reference for IVT (in vitro transcription) or LNP delivery systems.

eGFP mRNA (4 µg per 2 × 10⁶ cells; 30 min-5 d) electroporated into cord blood CD34⁺ cell-derived dendritic cells (under 1.75 kV/cm, 250 µs, single pulse conditions) achieves >90% transfection efficiency, induces sustained EGFP expression for up to 5 d, alters surface marker expression, and does not change allogeneic antigen-presenting capacity, with 97.7% cell viability post-electroporation^[1].
eGFP mRNA (4 µg per 2 × 10⁶ cells; 24 h) electroporated into peripheral blood adherent cell-derived dendritic cells (under 1.75 kV/cm, 250 µs, single pulse conditions) increases CD80, CD83, CD86, and HLA-DR surface expression without altering CD1a levels when analyzed 24 h post-transfection^[1].
eGFP mRNA (4 µg per 2 × 10⁶ cells; 24 h) electroporated into peripheral blood CD14+ cell-derived dendritic cells (under 1.75 kV/cm, 250 µs, single pulse conditions) slightly reduces CD1a expression, increases CD80, CD83, CD86, and HLA-DR expression, and does not change allogeneic antigen-presenting capacity when analyzed 24 h post-transfection^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

Shipping with dry ice.

-80°C

• [1]. Takahashi M, et al. Cytoplasmic expression of EGFP in dendritic cells transfected with in vitro transcribed mRNA or cellular total RNA extracted from EGFP expressing leukemia cells. Med Oncol. 2003;20(4):335-348.