F-P564
F-P564 is a FITC (HY-66019)-labeled HIF-1α-derived peptide substrate used to monitor PHD2 prolyl hydroxylase activity. F-P564 undergoes PHD2-mediated hydroxylation of Pro-564 to generate a hydroxyproline-containing peptide, which binds the VBC complex, increasing mass and enhancing fluorescence polarization in the assay. The unhydroxylated form of F-P564 shows no change in fluorescence polarization upon addition of VBC.
For research use only. We do not sell to patients.
- CAS No.: 869370-29-6
- Formula: C110H166TFIN25O35
- Molecular Weight:2547.56
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
PHD2 |
In Vitro
F-P564 (5 µM; 2 h) is hydroxylated by PHD2 in cell-free MALDI-TOF assays, producing a 2791-to-2807 Da mass shift that is modulated by CQ and Zn2+[1].
F-P564 (1 μM; 30 min) is hydroxylated by PHD2, producing a mass shift from 2793 to 2808 and enabling VBC binding in the fluorescence polarization assay[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 869370-29-6
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Molecular Weight 2547.56
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Formula C110H166TFIN25O35
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Sequence
FITC-Ahx-Asp-Leu-Asp-Leu-Glu-Ala-Leu-Ala-Pro-Tyr-Ile-Pro-Ala-Asp-Asp-Asp-Phe-Gln-Leu-Arg
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Sequence Shortening
FITC-Ahx-DLDLEALAPYIPADDDFQLR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Kim SY, et al. Effects of clioquinol analogues on the hypoxia-inducible factor pathway and intracelullar mobilization of metal ions. Biological & pharmaceutical bulletin. 2012;35(12):2160-9. [Content Brief]
[2]. Lee MJ, et al. Hinokitiol activates the hypoxia-inducible factor (HIF) pathway through inhibition of HIF hydroxylases. Biochemical and biophysical research communications. 2010 May 28;396(2):370-5. [Content Brief]
[3]. Cho H, et al. A fluorescence polarization-based interaction assay for hypoxia-inducible factor prolyl hydroxylases. Biochemical and biophysical research communications. 2005 Nov 11;337(1):275-80. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
F-P564 Related Classifications
HY-P12398 Related Classifications
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