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Cat. No.: HY-P3009

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Bovine Factor Xa

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Bovine Factor Xa is a serine protease that plays a central role in blood coagulation, with prothrombin as its physiological substrate. Bovine Factor Xa catalyzes the conversion of prothrombin to thrombin, activates factor VIII, and hydrolyzes peptide, thioester, and amide substrates. Bovine Factor Xa cleaves protease-activated receptors 1 and 2 to activate intracellular signal transduction. Bovine Factor Xa regulates multiple cellular pathways, mediates various cellular activities, tissue remodeling and cancer cell migration, and inhibits apoptosis in some cancer cells. Bovine Factor Xa protects hippocampal neurons against glutamate-induced damage. Bovine Factor Xa participates in embryonic vascular development and regulates immune hematopoiesis; its activity is inhibited by soybean trypsin inhibitor, and cannot be enhanced by phospholipids. Bovine Factor Xa regulates immune responses and macrophage polarization, and participates in fibrosis, vascular remodeling and tumor progression through non-coagulant effects. Bovine Factor Xa can be used in research related to atherosclerosis, fibrosis, asthma, cancer, thromboinflammation, sepsis, etc.

For research use only. We do not sell to patients.

CAS No.: 9002-05-5

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Please store the product under the recommended conditions in the Certificate of Analysis.

Bovine Factor Xa Chemical Structure

CAS No.: 9002-05-5

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Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Bovine Factor Xa (15-250 μM) and its Gla-domain truncated form (Factor Xa(-GD)) exhibit indistinguishable peptide p-nitroanilide hydrolytic activity, with nearly identical K_m and kcat values for both tested substrates^[2].
Bovine Factor Xa (0.16 μM; up to 60 minutes) and its Gla-domain truncated form (Factor Xa(-GD)) exhibit indistinguishable prothrombin activation activity in solution (with or without Factor Va), but only native bovine Factor Xa shows enhanced activity in the presence of phospholipid^[2].
Bovine Factor Xa retains full one-stage clotting activity when stored at 4 °C for 4.5 months in the presence of 5 mM CaCl₂, whereas activity drops to 20% of initial levels in the absence of CaCl₂^[2].
Bovine Factor Xa has chymotryptic proteolysis inhibited by divalent cations (Ca²⁺, Mg²⁺), with Ca²⁺ providing near-complete inhibition at concentrations >2 mM and a half-maximal inhibitory concentration of 0.6 mM^[2].
Bovine Factor Xa (5.5-55 nM) hydrolyzes a range of arginine-containing thioester substrates with highest specificity (k_cat/K_m up to 1.6 × 10⁵ M^-1s^-1) for substrates with a P₂ Gly residue, consistent with its natural prothrombin substrate^[4].
Bovine Factor Xa hydrolyzes Z-Trp-Arg-NA and Z-Trp-Arg-AMC amide substrates with k_cat/K_m values around 1.0 × 10⁴ M^-1s^-1, exhibiting unusual reactivity with amides compared to other coagulation factors^[4].
Bovine Factor Xa (5.5-55 nM) hydrolyzes select P'-extended thioester substrates with reduced reactivity (k_cat/K_m up to 4.5 × 10⁴ M^-1s^-1) relative to simple thioester substrates, showing a slight preference for the P₁' S-Ile analogue over S-Val^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 9002-05-5
Appearance Liquid
Color Colorless to light yellow
SMILES

[Bovine Factor Xa]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Borensztajn K, et al. Factor Xa: at the crossroads between coagulation and signaling in physiology and disease. Trends in molecular medicine. 2008 Oct;14(10):429-40. [Content Brief]

[2]. Morita T, et al. Preparation and properties of derivatives of bovine factor X and factor Xa from which the gamma-carboxyglutamic acid containing domain has been removed. The Journal of biological chemistry. 1986 Mar 25;261(9):4015-23.

[3]. Stenn KS, et al. Mechanism of bovine prothrombin activation by an insoluble preparation of bovine factor Xa (thrombokinase). Biochemistry. 1972 Nov 21;11(24):4502-15.

[4]. McRae BJ, et al. Mapping the active sites of bovine thrombin, factor IXa, factor Xa, factor XIa, factor XIIa, plasma kallikrein, and trypsin with amino acid and peptide thioesters: development of new sensitive substrates. Biochemistry. 1981 Dec 08;20(25):7196-206.

[5]. Ruf W, et al. Roles of factor Xa beyond coagulation. Journal of thrombosis and thrombolysis. 2021 Aug;52(2):391-396.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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