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Referencia número: HY-P11933

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FAHF

FAHF is a polypeptide-based dual-signal fluorescent colorimetric probe synthesized by conjugating the 5-carboxyfluorescein (5-FAM) (HY-66022) fluorophore to the Ala-His-Phe-NH₂ tripeptide backbone. FAHF achieves highly selective recognition of Cu²⁺ via fluorescence quenching and colorimetric change. The FAHF-Cu²⁺ complex responds to Glyphosate (HY-B0863) based on the displacement effect, resulting in fluorescence enhancement and a visually distinguishable color change. FAHF can penetrate cell membranes to enable cascade detection of copper (II) and glyphosate in living cells, and it can also be combined with smartphone RGB analysis for semi-quantitative identification.

Para uso exclusivo en investigación. No vendemos a pacientes.

Fòrmula: C₃₉H₃₄N₆O₉
Peso molecular:730.72

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Síntesis personalizada de péptidos

FAHF Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- In Vitro
Chemical Information
Pureza y Documentación
Referencias
Calculators
Help & FAQs
Classifications
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Actividad biológica

Descripciòn

In Vitro

FAHF (0-30.0 μM) is a selective, reversible, and rapid-response dual-signal probe for detecting Cu²⁺ and Glyphosate (HY-B0863) in 10 mM HEPES buffer (pH 7.4), with a limit of detection of 51.1 nM for Cu²⁺ and 46.8 nM for glyphosate^[1].
FAHF (0-80.0 μM; 24 h) exhibits low cytotoxicity against HaCaT cells, and can be used for cascade fluorescence imaging of Cu²⁺ and Glyphosate in living HaCaT cells^[1].
FAHF enables the detection of Cu²⁺ and Glyphosate in tap water and purified water; test strips based on FAHF allow naked-eye visual detection of Cu²⁺ and Glyphosate under ultraviolet light and natural light^[1].
FAHF (0-5.0 μM) enables smartphone-based RGB analysis for the semi-quantitative detection of Cu²⁺ and glyphosate, with limits of detection of 0.37 μM and 0.52 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

HaCaT cells
Concentration:

0, 5.0, 10.0, 20.0, 40.0, 80.0 μM
Incubation Time:

24 h
Result:

Maintained cell viability above 95% across all tested FAHF concentrations after 24 h.

Chemical Information

Peso molecular 730.72
Fòrmula C₃₉H₃₄N₆O₉
Sequence

{FAM}-Ala-His-Phe-NH2
Sequence Shortening

{FAM}-AHF-NH2
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Instrucciones de manejo (2659 KB)

Referencias

[1]. Wang P, et al. A novel peptide‑based fluorometric and colorimetric probe for cascade detection of copper(II) and glyphosate: Applications in real sample, cells imaging, test strips and smartphone‑assisted recognition. Journal of Molecular Structure, 2025, 1328: 141405.

Calculators

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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