FAHF
FAHF is a polypeptide-based dual-signal fluorescent colorimetric probe synthesized by conjugating the 5-carboxyfluorescein (5-FAM) (HY-66022) fluorophore to the Ala-His-Phe-NH2 tripeptide backbone. FAHF achieves highly selective recognition of Cu2+ via fluorescence quenching and colorimetric change. The FAHF-Cu2+ complex responds to Glyphosate (HY-B0863) based on the displacement effect, resulting in fluorescence enhancement and a visually distinguishable color change. FAHF can penetrate cell membranes to enable cascade detection of copper (II) and glyphosate in living cells, and it can also be combined with smartphone RGB analysis for semi-quantitative identification.
For research use only. We do not sell to patients.
- Formula: C39H34N6O9
- Molecular Weight:730.72
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
FAHF (0-30.0 μM) is a selective, reversible, and rapid-response dual-signal probe for detecting Cu2+ and Glyphosate (HY-B0863) in 10 mM HEPES buffer (pH 7.4), with a limit of detection of 51.1 nM for Cu2+ and 46.8 nM for glyphosate[1].
FAHF (0-80.0 μM; 24 h) exhibits low cytotoxicity against HaCaT cells, and can be used for cascade fluorescence imaging of Cu2+ and Glyphosate in living HaCaT cells[1].
FAHF enables the detection of Cu2+ and Glyphosate in tap water and purified water; test strips based on FAHF allow naked-eye visual detection of Cu2+ and Glyphosate under ultraviolet light and natural light[1].
FAHF (0-5.0 μM) enables smartphone-based RGB analysis for the semi-quantitative detection of Cu2+ and glyphosate, with limits of detection of 0.37 μM and 0.52 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HaCaT cells
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Concentration:0, 5.0, 10.0, 20.0, 40.0, 80.0 μM
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Incubation Time:24 h
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Result:Maintained cell viability above 95% across all tested FAHF concentrations after 24 h.
Chemical Information
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Molecular Weight 730.72
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Formula C39H34N6O9
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Sequence
{FAM}-Ala-His-Phe-NH2
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Sequence Shortening
{FAM}-AHF-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FAHF Related Classifications
HY-P11933 Related Classifications
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