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Cat. No.: HY-170462

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CSPD substrate (25 mM)

CSPD substrate (25 mM) is a chemiluminescent alkaline phosphatase substrate. CSPD substrate (25 mM) can be cleaved by alkaline phosphatase to generate a chemiluminescent signal for detecting the activity of this enzyme.

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CAS No.: 142849-53-4
Formule: C₁₈H₂₀ClNa₂O₇P
Masse moléculaire:460.75

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (292 KB)

CSPD substrate (25 mM) Chemical Structure

CAS No.: 142849-53-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
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Activité biologique

Description

In Vitro

CSPD substrate can be used to evaluate bone morphogenetic protein-2 (BMP-2)-induced osteogenic differentiation. In two osteoprogenitor cell lines, W20-17 and C3H10T1/2, the CSPD assay successfully monitors the upregulation of endogenous alkaline phosphatase activity following rhBMP-2 stimulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 142849-53-4
Masse moléculaire 460.75
Formule C₁₈H₂₀ClNa₂O₇P
SMILES

O=P(OC1=CC=CC(C2(OC)OOC23C4CC5CC3CC(C5)(C4)Cl)=C1)(O[Na])O[Na]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (292 KB)
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Blum JS, et al. An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2. J Cell Biochem. 2001;80(4):532-537. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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