Galanin Receptor Ligand M35

Name: Galanin Receptor Ligand M35
Brand: MedChemExpress
SKU: HY-P1840
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1840 Purity: 99.84%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Galanin Receptor Ligand M35 is a high-affinity ligand and antagonist of galanin receptor (K_d=0.1 nM). Galanin Receptor Ligand M35 exerts a K_i values of 0.11 and 2.0 nM for human galanin receptor type 1 and 2, respectively.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Galanin Receptor Ligand M35 Chemical Structure

CAS No. : 142846-71-7

Size	Price	Stock	Quantity
1 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Galanin Receptor Ligand M35:

Galanin Receptor Ligand M35 TFA Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Kd: 0.1 nM (galanin receptor)^[1]
Ki: 0.11 nM (human galanin receptor type 1), 2.0 nM (human galanin receptor type 2)^[2]

In Vitro

Galanin Receptor Ligand M35 has a dual effect on the galanin mediated inhibition of Forskolin stimulated cyclic AMP production in Rin m 5F cells. Co-applied with galanin (10 nM), Galanin Receptor Ligand M35 at low concentrations (1 nM) is able to reverse the inhibitory effect of galanin. But when present at higher concentrations (15 and 30 nM), Galanin Receptor Ligand M35 acts as a galanin receptor agonist, inhibiting the forskolin stimulated production of cAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Galanin Receptor Ligand M35 (6 nM/10 μL; i.c.v.) improves the ability of the male Sprague-Dnwley rats to acquire the swim maze task^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2233.60

Formula

C₁₀₇H₁₅₃N₂₇O₂₆

CAS No.

142846-71-7

Appearance

Solid

Color

White to off-white

Sequence

Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-NH2

Sequence Shortening

GWTLNSAGYLLGPPPGFSPFR-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (44.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (22.39 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4477 mL	2.2385 mL	4.4771 mL
5 mM	0.0895 mL	0.4477 mL	0.8954 mL
10 mM	0.0448 mL	0.2239 mL	0.4477 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.84%

Select Batch:

Data Sheet (270 KB) SDS (0 KB)

COA (242 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Kask K, et al. Binding and agonist/antagonist actions of M35, galanin(1-13)-bradykinin(2-9)amide chimeric peptide, in Rin m 5F insulinoma cells. Regul Pept. 1995 Nov 10;59(3):341-8. [Content Brief]

[2]. Webling KE, et al. Galanin receptors and ligands. Front Endocrinol (Lausanne). 2012 Dec 7;3:146. [Content Brief]

[3]. Ogren SO, et al. Evidence for a role of the neuropeptide galanin in spatial learning. Neuroscience. 1992 Nov;51(1):1-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.4477 mL	2.2385 mL	4.4771 mL	11.1927 mL
	5 mM	0.0895 mL	0.4477 mL	0.8954 mL	2.2385 mL
	10 mM	0.0448 mL	0.2239 mL	0.4477 mL	1.1193 mL
	15 mM	0.0298 mL	0.1492 mL	0.2985 mL	0.7462 mL
	20 mM	0.0224 mL	0.1119 mL	0.2239 mL	0.5596 mL
DMSO	25 mM	0.0179 mL	0.0895 mL	0.1791 mL	0.4477 mL
	30 mM	0.0149 mL	0.0746 mL	0.1492 mL	0.3731 mL
	40 mM	0.0112 mL	0.0560 mL	0.1119 mL	0.2798 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.