AGL-2263
Based on 1 publication(s) in Google Scholar
AGL-2263 is an insulin receptor and insulin-like growth factor (IGF) receptor inhibitor.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.31%
- No. CAS: 638213-98-6
- Fòrmula: C17H10N2O5
- Peso molecular:322.27
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) AGL-2263
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Actividad biológica
Insulin receptor[1].
AGL-2263 (5 μM, for 1 h followed by insulin treatment for 24 h) inhibits IR and insulin could still induce phosphorylation of AKT and ERK1/2 after the IR inhibition[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Granulosa cells.
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Concentration:5 μM.
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Incubation Time:For 1 h followed by insulin treatment for 24 h.
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Result:Insulin still induced phosphorylation of AKT and ERK1/2 after IR was inhibited by AGL2263.
Chemical Information
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No. CAS 638213-98-6
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Appearance Solid
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Peso molecular 322.27
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Fòrmula C17H10N2O5
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Color Light yellow to yellow
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SMILES
O=C(C1=CC(O)=C(O)C=C1)/C(C#N)=C/C2=CC=C(OC3=O)C(N3)=C2
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Reprod Biol
Insulin mitigates apoptosis of porcine follicular granulosa cells by downregulating BimEL. [Abstract]2019 Sep;19(3):293-298. PMID: 31561987
AGL-2263 purchased from MedChemExpress. Usage Cited in: Reprod Biol. 2019 Sep;19(3):293-298. [Abstract]
Granulosa cells pretreated with AG1024 (10 or 5 μM, inhibitor of IGF-1R autophosphorylation) for 1 h before insulin stimulation for 24 h. Levels of P-AKT, P-ERK 1/2, and β-actin detected in whole-cell lysates.
Solvente y solubilidad
DMSO : 100 mg/mL (310.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Li Y, et al. GDM-associated insulin deficiency hinders the dissociation of SERT from ERp44 and down-regulates placental 5-HT uptake. Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):E5697-705. [Content Brief]
[2]. Hua Zhang, et al. Somatic cells initiate primordial follicle activation and govern the development of dormant oocytes in mice. Curr Biol. 2014 Nov 3;24(21):2501-8. [Content Brief]
[3]. Ying Han, et al. Insulin mitigates apoptosis of porcine follicular granulosa cells by downregulating BimEL. Reprod Biol. 2019 Sep;19(3):293-298. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1030 mL | 15.5149 mL | 31.0299 mL | 77.5747 mL |
| 5 mM | 0.6206 mL | 3.1030 mL | 6.2060 mL | 15.5149 mL | |
| 10 mM | 0.3103 mL | 1.5515 mL | 3.1030 mL | 7.7575 mL | |
| 15 mM | 0.2069 mL | 1.0343 mL | 2.0687 mL | 5.1716 mL | |
| 20 mM | 0.1551 mL | 0.7757 mL | 1.5515 mL | 3.8787 mL | |
| 25 mM | 0.1241 mL | 0.6206 mL | 1.2412 mL | 3.1030 mL | |
| 30 mM | 0.1034 mL | 0.5172 mL | 1.0343 mL | 2.5858 mL | |
| 40 mM | 0.0776 mL | 0.3879 mL | 0.7757 mL | 1.9394 mL | |
| 50 mM | 0.0621 mL | 0.3103 mL | 0.6206 mL | 1.5515 mL | |
| 60 mM | 0.0517 mL | 0.2586 mL | 0.5172 mL | 1.2929 mL | |
| 80 mM | 0.0388 mL | 0.1939 mL | 0.3879 mL | 0.9697 mL | |
| 100 mM | 0.0310 mL | 0.1551 mL | 0.3103 mL | 0.7757 mL |
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