Autophinib
Based on 12 publication(s) in Google Scholar
Autophinib is a potent, selective autophagy inhibitor with IC50s of 90 nM and 40 nM for starvation- and Rapamycin-induced autophagy, respectively. Autophinib is also an ATP competitive Vacuolar Protein Sorting 34 (VPS34) inhibitor with an IC50 of 19 nM. Autophinib inhibits autophagy induced by starvation or Rapamycin by targeting VPS34.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.95%
- No. CAS: 1644443-47-9
- Fòrmula: C14H11ClN6O3
- Peso molecular:346.73
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Autophinib
More- J Extracell Vesicles. 2023 Apr;12(4):e12319. [Abstract]
- J Nanobiotechnology. 2025 Feb 14;23(1):109. [Abstract]
- Cell Death Dis. 2022 Aug 18;13(8):721. [Abstract]
- Cell Death Dis. 2019 Jan 17;10(2):41. [Abstract]
- Redox Rep. 2025 Dec;30(1):2588086. [Abstract]
- Cancer Sci. 2020 May;111(5):1542-1554. [Abstract]
- Fish Shellfish Immunol. 2023 Dec:143:109214. [Abstract]
- J Cancer. 2020 Mar 26;11(12):3655-3666. [Abstract]
- Allergy Asthma Clin Immunol. 2022 Jun 19;18(1):55. [Abstract]
- Vet Microbiol. 2021 Oct:261:109177. [Abstract]
- bioRxiv. 2025 Sep 21.
- Research Square Preprint. 2023 Jun 26.
Customer Validation & Images
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WB
Actividad biológica
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Vps34 19 nM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
0.04 μM
Compound: 45
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Inhibition of rapamycin induced autophagy in human MCF7 cells expressing eGFP-LC3
Inhibition of rapamycin induced autophagy in human MCF7 cells expressing eGFP-LC3
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[PMID: 36283182] |
| MCF7 | IC50 |
0.09 μM
Compound: 45
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Inhibition of starvation induced autophagy in human MCF7 cells expressing eGFP-LC3
Inhibition of starvation induced autophagy in human MCF7 cells expressing eGFP-LC3
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[PMID: 36283182] |
Autophinib (0.01-1 μM) inhibits LC3 lipidation to form LC3-II in a dose-dependent manner in starved MCF7-LC3 cells. Consistent with inhibition of autophagic flux, Autophinib also inhibits p62 degradation by autophagy dose-dependently in MCF7-LC3 cells[1].
Autophinib enhances cell death (EC50 of 264 nM) of starved cells as compared to fed cells, which occurred via the induction of apoptosis (EC50 of 234 nM) in MCF7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1644443-47-9
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Appearance Solid
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Peso molecular 346.73
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Fòrmula C14H11ClN6O3
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Color Light yellow to yellow
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SMILES
CC1=CC(NC2=NC(OC3=CC=C([N+]([O-])=O)C=C3)=NC(Cl)=C2)=NN1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (12)
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Journal Impact Factor
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Most Recent
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J Extracell Vesicles
Exosomal lipid PI4P regulates small extracellular vesicle secretion by modulating intraluminal vesicle formation. [Abstract]2023 Apr;12(4):e12319. PMID: 37021404 -
J Nanobiotechnology
Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity. [Abstract]2025 Feb 14;23(1):109. PMID: 39953537 -
Cell Death Dis
Whole transcriptome and proteome analyses identify potential targets and mechanisms underlying tumor treating fields against glioblastoma. [Abstract]2022 Aug 18;13(8):721. PMID: 35982032 -
Cell Death Dis
Increased expression of lncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma. [Abstract]2019 Jan 17;10(2):41. PMID: 30674868 -
Redox Rep
The novel thioredoxin reductase inhibitor butaselen suppresses lung cancer by inducing oxidative stress. [Abstract]2025 Dec;30(1):2588086. PMID: 41292488 -
Cancer Sci
Loss of the clock gene Per1 promotes oral squamous cell carcinoma progression via the AKT/mTOR pathway. [Abstract]2020 May;111(5):1542-1554. PMID: 32086839
Autophinib purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2020 May;111(5):1542-1554. [Abstract]
Western blotting shows that the decreased protein expression of P62 in Per1‐OE SCC15 cells is remarkably increased after the addition of Autophinib, while the increased LC3BII/LC3BI ratio and Beclin1 expression are remarkably decreased.
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Fish Shellfish Immunol
Butyrate induces STAT3/HIF-1α/IL-22 signaling via GPCR and HDAC3 inhibition to activate autophagy in head kidney macrophages from turbot (Scophthalmus maximus L.). [Abstract]2023 Dec:143:109214. PMID: 37977544 -
J Cancer
Overexpression of the clock gene Per2 suppresses oral squamous cell carcinoma progression by activating autophagy via the PI3K/AKT/mTOR pathway. [Abstract]2020 Mar 26;11(12):3655-3666. PMID: 32284762 -
Allergy Asthma Clin Immunol
2022 Jun 19;18(1):55. PMID: 35718777 -
Vet Microbiol
Lipid metabolism is a novel and practical source of potential targets for antiviral discovery against porcine parvovirus. [Abstract]2021 Oct:261:109177. PMID: 34391196 -
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Solvente y solubilidad
DMSO : 6.67 mg/mL (19.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.5 mg/mL (1.44 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
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- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8841 mL | 14.4204 mL | 28.8409 mL | 72.1022 mL |
| 5 mM | 0.5768 mL | 2.8841 mL | 5.7682 mL | 14.4204 mL | |
| 10 mM | 0.2884 mL | 1.4420 mL | 2.8841 mL | 7.2102 mL | |
| 15 mM | 0.1923 mL | 0.9614 mL | 1.9227 mL | 4.8068 mL |
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