Bathophenanthroline
Based on 1 Customer Validation
Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru2+. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.73%
- No. CAS: 1662-01-7
- Fòrmula: C24H16N2
- Peso molecular:332.40
-
Almacenamiento:
RT, protect from light, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
HY-D0005
BathocuproineCu(I) Chelating Agent -
HY-15902
ABTS diammonium saltHRP Substrate -
HY-W073017
Copper(II) Phthalocyanine (purified by sublimation)Metal-organic Framework -
HY-Y1841
o-Phenanthroline monohydrateMMP Inhibitor -
HY-D0017
Dansyl chlorideFluorescent Dye -
HY-W034953
Bathocuproine disulfonate disodiumBiochemical Assay Reagent -
HY-D0042
NBD-ClNonfluorescent Reagent -
HY-D1168
Oil Red ODiazol Dye -
HY-W076166C
Lead (II) iodideChemical Compound -
![2-[3,5-Bis(1-Phenylbenzimidazol-2-yl)phenyl]-1-phenylbenzimidazole](https://file.medchemexpress.com/product_pic/hy-w043044.gif)
HY-W043044
2-[3,5-Bis(1-Phenylbenzimidazol-2-yl)phenyl]-1-phenylbenzimidazoleMetal-organic Framework -
HY-15928
4-Nitrophenyl phosphate disodiumPhosphatases Substrate -
HY-B1422
9-AminoacridineAntimicrobial
Actividad biológica
Descripciòn
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>100 μM
Compound: GNF-Pf-4554
|
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
|
[PMID: 18579783] |
In Vitro
In SDS-PAGE gel staining experiments, heteroligand ruthenium(II) complexes containing bathophenanthroline (BP) and bathophenanthroline disulfonate (BPS) shows higher sensitivity than SYPRO Ruby for protein detection at near-neutral pH and reduces artificial post-translational modifications[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Fix gels in 7% acetic acid/10% ethanol.
2. Stain overnight with 0.5-1 mg/L Ru(BPS)2(BP) or Ru(BPS)(BP)2 in acidic/neutral buffer, destained.
3. Scan for fluorescence at 532 nm excitation/610 nm emission.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
No. CAS 1662-01-7
-
Appearance Solid
-
Peso molecular 332.40
-
Fòrmula C24H16N2
-
Color Off-white to light yellow
-
SMILES
C12=C(N=CC=C3C4=CC=CC=C4)C3=CC=C1C(C5=CC=CC=C5)=CC=N2
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
RT, protect from light, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Solvente y solubilidad
In Vitro:
DMSO : 2.78 mg/mL (8.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
-
Ficha de datos (275 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0084 mL | 15.0421 mL | 30.0842 mL | 75.2106 mL |
| 5 mM | 0.6017 mL | 3.0084 mL | 6.0168 mL | 15.0421 mL |
Bathophenanthroline Related Classifications
HY-D0006 Related Classifications
Powered by Bioz