CY3-SE
Based on 1 publication(s) in Google Scholar
Cy3-SE (Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester) is a sulfonated cyanine dye-derived fluorescent labeling reagent with λex of about 515 nm and λem of about 568 nm. Cy3-SE can interact with the π-π stacking of biomolecules (e.g., nucleoside monophosphates, proteins), inhibiting the photoisomerization process and increasing the fluorescence quantum yield and lifetime.
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- Pureza: 95.0%
- No. CAS: 146368-16-3
- Fòrmula: C35H41N3O10S2
- Peso molecular:727.84
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Almacenamiento:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CY3-SE
More
Actividad biológica
In the presence of nucleoside monophosphates (dNMPs) in solution, Cy3-SE inhibits photoisomerization through π-π stacking interactions, significantly increasing the fluorescence efficiency and lifetime. It has a better enhancement effect on purines (dAMP, dGMP) than on pyrimidines (dCMP, dTMP)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolution: Dissolve Cy3-SE in DMSO or other suitable organic solvents to prepare a stock solution;
2. Reaction: Dissolve the target molecule (e.g., peptide, antibody) in a buffer solution (e.g., 5 mM PBS, pH 8.0), add an equimolar or excess amount of Cy3-SE solution, and react at room temperature in the dark for 4-6 hours;
3. Purification: Remove unreacted Cy3-SE by dialysis (molecular weight cutoff 2000 Da) or high performance liquid chromatography (HPLC), and collect the labeled product.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
570
550
Chemical Information
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No. CAS 146368-16-3
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Appearance Solid
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Peso molecular 727.84
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Fòrmula C35H41N3O10S2
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Color Brown to red
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SMILES
CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5
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Synonyms
Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Pharmaceutics
The Influence of the External Chemistry of Silica-Based Mesoporous Nanocarriers on Organ Tropism and the Inhibition of Pulmonary Metastases. [Abstract]2025 Oct 26;17(11):1389. PMID: 41304726
Solvente y solubilidad
DMSO : 50 mg/mL (68.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Harvey BJ, et al. Nucleobase-specific enhancement of Cy3 fluorescence. J Fluoresc. 2009 May;19(3):443-8. [Content Brief]
[2]. Dang YQ, et al. Construction of a supramolecular Förster resonance energy transfer system and its application based on the interaction between Cy3-labeled melittin and phosphocholine encapsulated quantum dots. ACS Appl Mater Interfaces. 2012 Mar;4(3):1267-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3739 mL | 6.8696 mL | 13.7393 mL | 34.3482 mL |
| 5 mM | 0.2748 mL | 1.3739 mL | 2.7479 mL | 6.8696 mL | |
| 10 mM | 0.1374 mL | 0.6870 mL | 1.3739 mL | 3.4348 mL | |
| 15 mM | 0.0916 mL | 0.4580 mL | 0.9160 mL | 2.2899 mL | |
| 20 mM | 0.0687 mL | 0.3435 mL | 0.6870 mL | 1.7174 mL | |
| 25 mM | 0.0550 mL | 0.2748 mL | 0.5496 mL | 1.3739 mL | |
| 30 mM | 0.0458 mL | 0.2290 mL | 0.4580 mL | 1.1449 mL | |
| 40 mM | 0.0343 mL | 0.1717 mL | 0.3435 mL | 0.8587 mL | |
| 50 mM | 0.0275 mL | 0.1374 mL | 0.2748 mL | 0.6870 mL | |
| 60 mM | 0.0229 mL | 0.1145 mL | 0.2290 mL | 0.5725 mL |
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