ST271
Based on 1 Customer Validation
ST271 is a potent inhibitor of protein tyrosine kinase (PTK), inhibits phospholipase D activation stimulated by fMet-Leu-Phe and PAF, with IC50s of 6.7 and 9 μM, respectively.
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- Pureza: 98.00%
- No. CAS: 106392-48-7
- Fòrmula: C16H20N2O2
- Peso molecular:272.34
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
IC50: 6.7 μM (phospholipase D, stimulated by fMet-Leu-Phe), 9 μM (phospholipase D, stimulated by PAF)[3]
ST271 partially inhibits peptide phosphorylation in the membrane preparation and in permeabilized platelets[1]. ST271 (100 μM) causes complete inhibition of formation of inositol phosphates induced by FcγRII cross-linking, but also induces a small (< 30%) but significant inhibition of the response to thrombin and U46619[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 106392-48-7
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Appearance Solid
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Peso molecular 272.34
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Fòrmula C16H20N2O2
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Color Light yellow to yellow
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SMILES
O=C(N)/C(C#N)=C/C1=CC(C(C)C)=C(O)C(C(C)C)=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 50 mg/mL (183.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Martinson EA, et al. Inhibition of phospholipase D of human platelets by protein tyrosine kinase inhibitors. Cell Mol Biol (Noisy-le-grand). 1994 Jul;40(5):627-34. [Content Brief]
[2]. Blake RA, et al. Fc gamma receptor II stimulated formation of inositol phosphates in human platelets is blocked by tyrosine kinase inhibitors and associated with tyrosine phosphorylation of the receptor. [Content Brief]
[3]. Uings IJ, et al. Tyrosine phosphorylation is involved in receptor coupling to phospholipase D but not phospholipase C in the human neutrophil. Biochem J. 1992 Feb 1;281 (Pt 3):597-600. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6719 mL | 18.3594 mL | 36.7188 mL | 91.7970 mL |
| 5 mM | 0.7344 mL | 3.6719 mL | 7.3438 mL | 18.3594 mL | |
| 10 mM | 0.3672 mL | 1.8359 mL | 3.6719 mL | 9.1797 mL | |
| 15 mM | 0.2448 mL | 1.2240 mL | 2.4479 mL | 6.1198 mL | |
| 20 mM | 0.1836 mL | 0.9180 mL | 1.8359 mL | 4.5899 mL | |
| 25 mM | 0.1469 mL | 0.7344 mL | 1.4688 mL | 3.6719 mL | |
| 30 mM | 0.1224 mL | 0.6120 mL | 1.2240 mL | 3.0599 mL | |
| 40 mM | 0.0918 mL | 0.4590 mL | 0.9180 mL | 2.2949 mL | |
| 50 mM | 0.0734 mL | 0.3672 mL | 0.7344 mL | 1.8359 mL | |
| 60 mM | 0.0612 mL | 0.3060 mL | 0.6120 mL | 1.5300 mL | |
| 80 mM | 0.0459 mL | 0.2295 mL | 0.4590 mL | 1.1475 mL | |
| 100 mM | 0.0367 mL | 0.1836 mL | 0.3672 mL | 0.9180 mL |
ST271 Related Classifications
HY-103097 Related Classifications
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