Substance P (7-11)
Based on 1 Customer Validation
Substance P (7-11) is a C-terminal fragment of Substance P which can cause an increase in the intracellular calcium concentration.
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- Pureza: 99.08%
- No. CAS: 51165-05-0
- Fòrmula: C31H44N6O5S
- Peso molecular:612.78
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Almacenamiento:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Substance P (7-11) increases PGE2 and collagenase production at concentrations greater than 1 μM. Substance P (7-11), but not intact SP, SP-(1-4), SP-(1-6), SP- (8-11) or SP-(9-1 1), nor the tachykinins NKA and NKB, causes an increase in the intracellular calcium concentration as measured by the fluorescent dye Fura-2. The maximal change in intracellular calcium induced by 10 μM Substance P (7-11) was 140±30 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 51165-05-0
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Appearance Solid
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Peso molecular 612.78
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Fòrmula C31H44N6O5S
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Color White to off-white
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Sequence
Phe-Phe-Gly-Leu-Met-NH2
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Sequence Shortening
FFGLM-NH2
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvente y solubilidad
H2O : 1.96 mg/mL (3.20 mM; ultrasonic and adjust pH to 2 with HCl)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
Confluent chondrocytes are incubated for 24 h with SP or Substance P (7-11). TIMP activity is inactivated following reduction and alkylation with 2 mM dithiothreitol and 5 mM iodoacetamide for 30 min at 37°C. Samples are subsequently dialysed. Latent collagenase is activated with 1 mM APt followed by inactivation with soy bean trypsin inhibitor. Collagenase is measured[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.6319 mL | 8.1595 mL | 16.3191 mL | 40.7977 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Substance P (7-11) Related Classifications
HY-P1492 Related Classifications
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