BF6
BF6 is a blood-brain barrier permeable NIR probe. BF6 can diffuse from intracranial blood vessels into brain tissue, enabling visualization of brain structures and differentiation between glioblastoma (GBM) and normal tissues. The absorption peaks of BF6 in DMSO and FBS range from 727 to 859 nm, with a broad fluorescence emission band spanning 800 to 1400 nm; its emission wavelength falls within the NIR-II region (800-1400 nm).
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- Fòrmula: C20H22BF2N7O
- Peso molecular:425.24
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Actividad biológica
Operating Instructions (The following is a recommended experimental protocol for guidance only; please adjust it according to your specific requirements).
1. Staining Procedure[1]
1.1 Sample Type: Adherent cells (U-87 MG cells)
1.1.1 Adherent cells: Trypsin digestion is not required for the staining procedure.
1.2 Incubation Conditions: Incubate with 2 μM BF6 for 20 min; use 5 μM BF6 for incubation in an in vitro blood-brain barrier (BBB) model.
1.3 Washing Step: Wash the cells after incubation to remove excess dye.
2. Control Setup
2.1 Evans Blue serves as the negative control for in vitro BBB permeability assays.
3. Detection and Analysis
3.1 Instrument: NIR-II microscope.
3.1.1 Cell Imaging Parameters: Ex = 663 nm, Em = 950 nm.
3.2 Result Analysis:
3.2.1 Fluorescence Intensity Change: Bright NIR-II fluorescence is exhibited in the cellular environment.
3.2.2 Fluorescence Localization: Targets intracellular lipid droplets.
3.2.3 Color Change: Presents pseudo-colored NIR-II fluorescence with a gradient from low to high intensity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
BF6 (200 μM; tail vein injection) distinguishes normal brain tissue from glioblastoma tissue in mouse models, exhibits higher fluorescence intensity in normal tissues, and enables clear delineation of tumor boundaries via NIR-II imaging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic nude mice[1]
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Dosage:200 μM
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Administration:i.v. via tail vein
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Result:Detected significant NIR-II fluorescence signals in brain tissue after injection.
Reached maximum fluorescence intensity at 45 min post-injection, with a signal-to-noise ratio range of 2.5-2.9.
Showed significant accumulation in brain tissue via ex vivo imaging at 2 h post-injection.
Detected a peak at m/z + H = 426.2 (corresponding to BF6) in brain homogenates via mass spectrometry.
Confirmed accumulation in brain tissue via HPLC analysis.
Increased the NIR-II fluorescence ratio between brain tissue and vessels within 160 s, with tissue intensity overtaking vessel intensity at 100 s post-injection.
Exhibited negligible toxicity, with H&E staining of major organs collected 1 month post-injection showing negligible toxicity compared to PBS-treated mice.
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Animal Model:Athymic nude mice (intracranial implantation of U-87 MG GBM cells, allowed to grow for 3-4 weeks)[1]
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Dosage:200 μM
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Administration:i.v. via tail vein
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Result:
Showed uneven brain fluorescence imaging after injection, with fluorescence intensity of partial brain tissues increasing from 0 to 60 min.
Mirrored in vivo results via ex vivo imaging.
Exhibited higher fluorescence intensity in normal brain tissue than glioma tissue, allowing clear differentiation and delineation of tumor margins.
Achieved a normal-to-glioma fluorescence ratio significantly larger than that observed with ICG, indicating superior differentiation capability.
Chemical Information
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Peso molecular 425.24
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Fòrmula C20H22BF2N7O
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SMILES
CN(C1=CC=C(C=C1)[N+]2=NC(C#N)=NN([B-]2(F)F)C3=CC=C(C=C3)N4CCOCC4)C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BF6 Related Classifications
HY-D3113 Related Classifications
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