Ebeiedinone
Based on 1 Customer Validation
Ebeiedinone, a steroidal alkaloid from Fritillaria species, inhibits the bioactivity of human whole blood cholinesterase (ChE) at the concentration of 0.1 mM, with the inhibitory effects of 69.0%.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.90%
- No. CAS: 25650-68-4
- Fòrmula: C27H43NO2
- Peso molecular:413.64
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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AChE |
BChE |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | CC50 |
22 μM
Compound: 6
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Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
| NCI-H460 | CC50 |
37 μM
Compound: 6
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Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
Chemical Information
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No. CAS 25650-68-4
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Appearance Solid
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Peso molecular 413.64
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Fòrmula C27H43NO2
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Color Off-white to light yellow
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SMILES
C[C@@]([C@]1([H])C[C@H]2O)(CC2)[C@]3([H])[C@](CC1=O)([H])[C@@](CC[C@@]4([H])[C@@]5([H])CN(C[C@H]6C)[C@](CC6)([H])[C@@H]4C)([H])[C@@]5([H])C3
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 100 mg/mL (241.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.04 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4176 mL | 12.0878 mL | 24.1756 mL | 60.4390 mL |
| 5 mM | 0.4835 mL | 2.4176 mL | 4.8351 mL | 12.0878 mL | |
| 10 mM | 0.2418 mL | 1.2088 mL | 2.4176 mL | 6.0439 mL | |
| 15 mM | 0.1612 mL | 0.8059 mL | 1.6117 mL | 4.0293 mL | |
| 20 mM | 0.1209 mL | 0.6044 mL | 1.2088 mL | 3.0220 mL | |
| 25 mM | 0.0967 mL | 0.4835 mL | 0.9670 mL | 2.4176 mL | |
| 30 mM | 0.0806 mL | 0.4029 mL | 0.8059 mL | 2.0146 mL | |
| 40 mM | 0.0604 mL | 0.3022 mL | 0.6044 mL | 1.5110 mL | |
| 50 mM | 0.0484 mL | 0.2418 mL | 0.4835 mL | 1.2088 mL | |
| 60 mM | 0.0403 mL | 0.2015 mL | 0.4029 mL | 1.0073 mL | |
| 80 mM | 0.0302 mL | 0.1511 mL | 0.3022 mL | 0.7555 mL | |
| 100 mM | 0.0242 mL | 0.1209 mL | 0.2418 mL | 0.6044 mL |
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