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Ethidium bromide (solution)

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Ethidium bromide (solution) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Solvent and Concentration: Sterile water: 10 mg/Ml

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No. CAS: 1239-45-8
Fòrmula: C₂₁H₂₀BrN₃
Peso molecular:394.31

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Ethidium bromide (solution) Chemical Structure

No. CAS: 1239-45-8

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
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- Cellular Effect
- In Vitro
Chemical Information
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Actividad biológica

Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.1 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma A2780 cell lines Concentration required to inhibit 50% of human carcinoma A2780 cell lines	[PMID: 15501053]
CH1	IC₅₀	0.4 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma CH1 cell lines Concentration required to inhibit 50% of human carcinoma CH1 cell lines	[PMID: 15501053]
MDA-MB-231	IC₅₀	2.33 μM Compound: Ethidium bromide	Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25965778]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA	[PMID: 24119869]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25554922]
MGC-803	IC₅₀	2.18 μM Compound: 2	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method	[PMID: 26883149]
MGC-803	IC₅₀	2.39 μM Compound: Ethidium bromide	Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 23265905]
MGC-803	IC₅₀	2.47 μM Compound: Ethidium bromide	Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 22405648]
NIH3T3	IC₅₀	134.87 μM Compound: Ethidium bromide	Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay	[PMID: 24286761]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20800480]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20947362]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP	[PMID: 21486698]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 23312949]
SK-OV-3	IC₅₀	4.5 μM Compound: Ethidium	Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines Concentration required to inhibit 50% of human carcinoma SKOV3 cell lines	[PMID: 15501053]
SW 1116	IC₅₀	2.71 μM Compound: Ethidium bromide	Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 24286761]

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1 X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).
4. Make the solution cool down before solidification.
5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/mL, and require 5 μL stock/100 mL gel).
6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

No. CAS 1239-45-8
Appearance Liquid (Density: 1.3739 g/cm³)
Peso molecular 394.31
Fòrmula C₂₁H₂₀BrN₃
Color Brown to red
SMILES

CC[N+]1=C(C2=CC(N)=CC=C2C3=C1C=C(C=C3)N)C4=CC=CC=C4.[Br-]
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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Biol Reprod

O-GlcNAcylation of p66Shc drives senescence associated secretory phenotype via mitochondrial DNA release in gestational diabetes mellitus†. [Abstract]

2026 May 11:ioag090. PMID: 42113624

Pureza y Documentación

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Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (274 KB)
SDS (251 KB)
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- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
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- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [Content Brief]

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

Calculators

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Calculadora de dilución

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Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Ethidium bromide (solution)

1 Cited Publications

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Ethidium bromide (solution)

Publications Citing Use of MedChemExpress (MCE) Ethidium bromide (solution)

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Ethidium bromide (solution) Related Classifications

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