JOE
Based on 1 Customer Validation
JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing.
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- Pureza: 96.36%
- No. CAS: 82855-40-1
- Fòrmula: C23H14Cl2O9
- Peso molecular:505.26
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
The fluorescence quantum yield of JOE (in 1× TBE/7 M urea solution) relative to fluorescein is 0.58, and its emission intensity is lower than that of the F10J ET primer when excited at 488 nm or 514 nm[2].
JOE-labeled molecular beacons exhibit higher fluorescence signals in the hairpin state than in the double-stranded state formed with complementary target ODN, and the signal levels vary with different probe types and linkers[3].
When the JOE-labeled probe forms a hairpin state and a double-stranded state with the complementary target ODN, it exhibits distinct fluorescence melting curve characteristics, thus enabling the differentiation of these two conformations[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 82855-40-1
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Appearance Solid
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Peso molecular 505.26
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Fòrmula C23H14Cl2O9
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Color Brown to reddish brown
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SMILES
O=C(C1=CC(C2(C3=C(OC4=C2C=C(OC)C(O)=C4Cl)C(Cl)=C(O)C(OC)=C3)O5)=C(C=C1)C5=O)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 50 mg/mL (98.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (9.90 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Tsybulsky DA, et al. 4',5'-Dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (JOE): synthesis and spectral properties of oligonucleotide conjugates. J Org Chem. 2012;77(2):977-984. [Content Brief]
[2]. Ju J, et al. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis. Proc Natl Acad Sci U S A. 1995;92(10):4347-4351. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.9792 mL | 9.8959 mL | 19.7918 mL | 49.4795 mL |
| 5 mM | 0.3958 mL | 1.9792 mL | 3.9584 mL | 9.8959 mL | |
| DMSO | 10 mM | 0.1979 mL | 0.9896 mL | 1.9792 mL | 4.9479 mL |
| 15 mM | 0.1319 mL | 0.6597 mL | 1.3195 mL | 3.2986 mL | |
| 20 mM | 0.0990 mL | 0.4948 mL | 0.9896 mL | 2.4740 mL | |
| 25 mM | 0.0792 mL | 0.3958 mL | 0.7917 mL | 1.9792 mL | |
| 30 mM | 0.0660 mL | 0.3299 mL | 0.6597 mL | 1.6493 mL | |
| 40 mM | 0.0495 mL | 0.2474 mL | 0.4948 mL | 1.2370 mL | |
| 50 mM | 0.0396 mL | 0.1979 mL | 0.3958 mL | 0.9896 mL | |
| 60 mM | 0.0330 mL | 0.1649 mL | 0.3299 mL | 0.8247 mL | |
| 80 mM | 0.0247 mL | 0.1237 mL | 0.2474 mL | 0.6185 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
JOE Related Classifications
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