PE-Cy5
PE-Cy5 is a tandem fluorescent dye commonly used in flow cytometry, immunofluorescence, and cell biology research. It is formed by the covalent linkage of two fluorescent molecules, namely phycoerythrin and CY5. PE-Cy5 binds to human FcγRI (CD64), and this binding can be blocked by human pooled serum, anti-CD64 monoclonal antibodies targeting the ligand-binding region, or aggregated IgG. PE-Cy5 supports three-color flow cytometry analysis, and whole blood staining can partially reduce its non-specific binding (Ex/Em = 450-500 nm/665 nm).
Para uso exclusivo en investigación. No vendemos a pacientes.
-
Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
PE-Cy5 conjugate specifically binds to cells expressing human FcγRI/CD64, including primary human monocytes, G-CSF-treated neutrophils, IFNγ-stimulated U937 cells, and FcγRI-transfected IIA1.6 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
SMILES
[PE-Cy5]
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. van Vugt MJ, van den Herik-Oudijk IE, et al. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996 Sep 15;88(6):2358-61. [Content Brief]
[2]. Hulspas R, et al. Flow cytometry and the stability of phycoerythrin-tandem dye conjugates. Cytometry A. 2009;75(11):966-972. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PE-Cy5 Related Classifications
HY-D3274 Related Classifications
Powered by Bioz
- PE-Cy5
- Fluorescent Dye
- acute myeloid leukemia-fab m3
- anti-CD64 monoclonal antibodies
- G-CSF-treated patient neutrophils
- IFNγ-stimulated U937 cells
- primary human monocytes
- human pooled serum
- aggregated IgG
- human peripheral blood mononuclear cells
- human FcγRI (CD64)
- FcγRI-transfected IIA1.6 cells
- Inhibitor
- inhibitor
- inhibit