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Referencia número: HY-118837

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WF-536

WF-536 is an orally active inhibitor of Rho-associated coiled-coil-containing protein kinase (ROCK). WF-536 has tumor anti-metastatic activity. WF-536 can be used for the research of cancer.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS: 539857-64-2
Fòrmula: C₁₄H₁₆ClN₃O
Peso molecular:277.75

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

WF-536 Chemical Structure

No. CAS: 539857-64-2

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Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
Chemical Information
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Calculators
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Actividad biológica

Descripciòn

WF-536 is an orally active inhibitor of Rho-associated coiled-coil-containing protein kinase (ROCK). WF-536 has tumor anti-metastatic activity. WF-536 can be used for the research of cancer^[1].

Ver todos los productos específicos de isoformas ROCK :

In Vitro

WF-536 (1, 3, 10, 30, 100 μM) inhibits both invasion and migration by LLC cells and invasion, migration, and formation of capillarylike tubes on Matrigel by endothelial cells, without cytotoxicity or anti-proliferative action in either cell type^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay ^[1]

Cell Line:

LLC cells, Human umbilical vein endothelial cells
Concentration:

1, 3, 10, 30 μM
Incubation Time:

6 h (tumor cells), 4 h (endothelial cells)
Result:

Significantly inhibited LLC-cell migration, with inhibitory rates of 23% and 44% at 10 and 30 μM, respectively.

Cell Invasion Assay^[1]

Cell Line:

LLC cells, Human umbilical vein endothelial cells
Concentration:

1, 3, 10, 30 μM
Incubation Time:

18 h
Result:

Showed significant and concentration-dependent inhibition of LLC-cell invasion.

Cell Proliferation Assay^[1]

Cell Line:

LLC cells, Human umbilical vein endothelial cells
Concentration:

1, 30, 100 μM
Incubation Time:

24, 48, 72, and 96 h
Result:

Showed no significant effect at 1-30 μM on the proliferation up to 96 h incubation, although the proliferation was decreased at 100 μM over 48 h.

In Vivo

WF-536 (oral; 0.3-3 mg/kg/day; for 28 days) inhibits Lewis lung carcinoma (LLC) metastasis and LLC-induced angiogenesis in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice (male, 6-week old)^[1]
Dosage:

0.3, 1, 3 mg/kg
Administration:

Oral; daily; for 28 days
Result:

Significantly reduced the number of pulmonary metastatic colonies of LLC in a dose-dependent manner (0.3- 3 mg/kg).

Chemical Information

No. CAS 539857-64-2
Peso molecular 277.75
Fòrmula C₁₄H₁₆ClN₃O
SMILES

O=C(C1=CC=C(C=C1)[C@@H](C)N)NC2=CC=NC=C2.Cl
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Nakajima, Masahide et al. Wf-536 prevents tumor metastasis by inhibiting both tumor motility and angiogenic actions. European journal of pharmacology vol. 459,2-3 (2003): 113-20. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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WF-536

Ver todos los productos específicos de isoformas ROCK

Actividad biológica

Chemical Information

Pureza y Documentación

Referencias

Calculators

Help & FAQs

WF-536 Related Classifications

HY-118837 Related Classifications