Gemcitabine monophosphate (Synonyms: Gemcitabine 5′-phosphate)

Name: Gemcitabine monophosphate
Brand: MedChemExpress
SKU: HY-108932
Rating: 4.5 (1 reviews)

Cat. No.: HY-108932 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Gemcitabine monophosphate (Gemcitabine 5′-phosphate) is one of the active intermediates of Gemcitabine (HY-17026). Gemcitabine monophosphate shows synergistic anti-cancer effects.

For research use only. We do not sell to patients.

Gemcitabine monophosphate Chemical Structure

CAS No. : 116371-67-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 528	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 528	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Gemcitabine monophosphate (Gemcitabine 5′-phosphate) is one of the active intermediates of Gemcitabine (HY-17026). Gemcitabine monophosphate shows synergistic anti-cancer effects^[1]^[2].

Molecular Weight

343.18

Formula

C₉H₁₂F₂N₃O₇P

CAS No.

116371-67-6

Appearance

Solid

Color

White to off-white

SMILES

OP(OC[C@@H]1[C@@H](O)C(F)(F)[C@H](N2C=CC(N)=NC2=O)O1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (582.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (291.39 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9139 mL	14.5696 mL	29.1392 mL
5 mM	0.5828 mL	2.9139 mL	5.8278 mL
10 mM	0.2914 mL	1.4570 mL	2.9139 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.57 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.57 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (14.57 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) HNMR (588 KB) CNMR (530 KB) RP-HPLC (310 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang Y, et al. Codelivery of VEGF siRNA and gemcitabine monophosphate in a single nanoparticle formulation for effective treatment of NSCLC. Mol Ther. 2013 Aug;21(8):1559-69. [Content Brief]

[2]. Miao L, et al. Nanoparticles with Precise Ratiometric Co-Loading and Co-Delivery of Gemcitabine Monophosphate and Cisplatin for Treatment of Bladder Cancer. Adv Funct Mater. 2014 Nov 12;24(42):6601-6611. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9139 mL	14.5696 mL	29.1392 mL	72.8481 mL
	5 mM	0.5828 mL	2.9139 mL	5.8278 mL	14.5696 mL
	10 mM	0.2914 mL	1.4570 mL	2.9139 mL	7.2848 mL
	15 mM	0.1943 mL	0.9713 mL	1.9426 mL	4.8565 mL
	20 mM	0.1457 mL	0.7285 mL	1.4570 mL	3.6424 mL
	25 mM	0.1166 mL	0.5828 mL	1.1656 mL	2.9139 mL
	30 mM	0.0971 mL	0.4857 mL	0.9713 mL	2.4283 mL
	40 mM	0.0728 mL	0.3642 mL	0.7285 mL	1.8212 mL
	50 mM	0.0583 mL	0.2914 mL	0.5828 mL	1.4570 mL
	60 mM	0.0486 mL	0.2428 mL	0.4857 mL	1.2141 mL
	80 mM	0.0364 mL	0.1821 mL	0.3642 mL	0.9106 mL
	100 mM	0.0291 mL	0.1457 mL	0.2914 mL	0.7285 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Gemcitabine monophosphate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gemcitabine monophosphate116371-67-6Gemcitabine 5′-phosphateApoptosisnon-small–cell lung cancerNSCLCVEGF siRNACisplatinbladder cancerInhibitorinhibitorinhibit

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Gemcitabine monophosphate (Synonyms: Gemcitabine 5′-phosphate)

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Complete Stock Solution Preparation Table

Gemcitabine monophosphate Related Classifications

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