Hspa5 - heat shock protein 5 Gene

Also Known as Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; D2Wsu17e; D2Wsu141e

Species: Mus musculus

Gene Type: protein coding

Gene ID: 14828

About Hspa5

Chromosome 2: 34,661,982-34,667,559 forward strand.GRCm39:CM000995.3

This gene has 6 transcripts (splice variants), 257 orthologues, 12 paralogues and is associated with 53 phenotypes. Ubiquitous expression in placenta adult (RPKM 321.6), genital fat pad adult (RPKM 154.1) and 28 other tissues.

Summary

Enables misfolded protein binding activity and ribosome binding activity. Involved in several processes, including IRE1-mediated unfolded protein response; cerebellum development; and positive regulation of protein ubiquitination. Acts upstream of or within several processes, including cellular response to interleukin-4; negative regulation of transforming growth factor beta receptor signaling pathway; and toxin transport. Located in several cellular components, including cell surface; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment. Part of endoplasmic reticulum chaperone complex. Is expressed in several structures, including alimentary system; brain; eye; genitourinary system; and musculoskeletal system. Human ortholog(s) of this gene implicated in dopamine beta-hydroxylase deficiency. Orthologous to human HSPA5 (heat shock protein family A (HSP70) member 5). [provided by Alliance of Genome Resources, Apr 2022]

Hspa5 Products (2)

mRNA	Protein	Name
NM_022310.3	NP_071705.3	endoplasmic reticulum chaperone BiP precursor
NM_001163434.1	NP_001156906.1	endoplasmic reticulum chaperone BiP precursor

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables misfolded protein binding	IDA IDA: Inferred from direct assay	18923430	MGI
enables protein binding	IPI IPI: Inferred from physical interaction	11836248	MGI
enables protein folding chaperone	IDA IDA: Inferred from direct assay	23123197	MGI
enables ribosome binding	IDA IDA: Inferred from direct assay	12065409	MGI

Biological Process GO Annotation	Evidence	References	Source
acts upstream of or within ER overload response	IDA IDA: Inferred from direct assay	11854325	MGI
involved in IRE1-mediated unfolded protein response	IEP IEP: Inferred from expression pattern	24508390	MGI
acts upstream of or within cellular response to interleukin-4	IDA IDA: Inferred from direct assay	9798653	MGI
involved in cerebellar Purkinje cell layer development	IMP IMP: Inferred from mutant phenotype	19816510	MGI
involved in cerebellum structural organization	IMP IMP: Inferred from mutant phenotype	19816510	MGI
involved in endoplasmic reticulum unfolded protein response	IMP IMP: Inferred from mutant phenotype	19816510	MGI
acts upstream of or within negative regulation of transforming growth factor beta receptor signaling pathway	IGI IGI: Inferred from genetic interaction	17991893	MGI
involved in positive regulation of protein ubiquitination	IMP IMP: Inferred from mutant phenotype	19816510	MGI
involved in proteolysis involved in protein catabolic process	IDA IDA: Inferred from direct assay	18923430	MGI

Cellular Component GO Annotation	Evidence	References	Source
located in cell surface	IDA IDA: Inferred from direct assay	17991893	MGI
located in endoplasmic reticulum	IDA IDA: Inferred from direct assay	7679955	MGI
part of endoplasmic reticulum chaperone complex	IDA IDA: Inferred from direct assay	12475965	MGI
located in endoplasmic reticulum lumen	IDA IDA: Inferred from direct assay	19816510	MGI
located in endoplasmic reticulum membrane	IDA IDA: Inferred from direct assay	22589549	MGI
located in endoplasmic reticulum-Golgi intermediate compartment	IDA IDA: Inferred from direct assay	21187406	MGI
located in plasma membrane	IDA IDA: Inferred from direct assay	22837305	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Hspa5 Protein Structure

HSP70

0
200
400
600
655 a.a.

Protein Preferred Names	Protein Names
endoplasmic reticulum chaperone BiP	78 kDa glucose-regulated protein GRP-78 HSP70 family protein 5 XAP-1 antigen binding-immunoglobulin protein glucose regulated protein, 78 kDa heat shock 70 kDa protein 5 heat shock 70kD protein 5 (glucose-regulated protein, 78kD) heat shock protein 70 family protein 5 heat shock protein family A member 5 immunoglobulin heavy chain-binding protein

Recombinant Hspa5 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P71742	HSPA5/GRP-78 Protein, Mouse (P.pastoris, His)	P20029 (E20-L655)	≥ 90%, as determined by reducing SDS-PAGE.
HY-P71742Y	HSPA5/GRP-78 Protein, Mouse (P.pastoris, His, solution)	P20029 (E20-L655)	≥ 90%, as determined by reducing SDS-PAGE.

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-W250122	Glutamic acid sodium salt	Activator	99.92%	Phase 1

Pre-clinical Phase

Bioactive Molecules (4)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-125974	HM03	Inhibitor	99.51%	Unkown
HY-152153	GRP78-IN-3	Inhibitor	99.88%	Unkown
HY-125974A	HM03 trihydrochloride	Inhibitor	/	Unkown
HY-184982	Estrogen receptor-IN-2	Activator	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Hspa5	NCBI	NCBI:3309