PRIM2 - DNA primase subunit 2 Gene

Also Known as p58; PRIM2A

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 5558

About PRIM2

Cytogenetic location: 6p11.2 Genomic coordinates (GRCh38): 6:57,221,540-57,646,850 (from NCBI)

This gene has 9 transcripts (splice variants) and 217 orthologues. Broad expression in prostate (RPKM 6.3), lymph node (RPKM 1.3) and 21 other tissues.

Summary

This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]

PRIM2 Products (3)

mRNA	Protein	Name
NM_000947.5	NP_000938.2	DNA primase large subunit isoform a
NM_001282487.2	NP_001269416.1	DNA primase large subunit isoform b
NM_001282488.2	NP_001269417.1	DNA primase large subunit isoform b

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables 4 iron, 4 sulfur cluster binding	IDA IDA: Inferred from direct assay	25550159	GOA
enables DNA/RNA hybrid binding	IDA IDA: Inferred from direct assay	26975377	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	20643958	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA replication initiation	IDA IDA: Inferred from direct assay	26975377	GOA
involved in DNA replication, synthesis of primer	IDA IDA: Inferred from direct assay	8026492	GOA
involved in positive regulation of DNA primase activity	IMP IMP: Inferred from mutant phenotype	25550159	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of alpha DNA polymerase:primase complex	IDA IDA: Inferred from direct assay	8026492	GOA
part of alpha DNA polymerase:primase complex	IPI IPI: Inferred from physical interaction	26975377	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PRIM2 Protein Structure

DNA_primase_lrg

0
100
200
300
346 a.a.

Protein Preferred Names	Protein Names
DNA primase large subunit	DNA primase 58 kDa subunit

PRIM2 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	PRIM2	P49643	RECK	Homo sapiens	Q6P9E2	Validated Y2H	32296183
Intra	PRIM2	P49643	SPRED1	Homo sapiens	Q7Z699	Validated Y2H	32814053
Intra	PRIM2	P49643	SPRED1	Homo sapiens	Q7Z699	Y2H Pooling	32814053
Intra	PRIM2	P49643	SPRED1	Homo sapiens	Q7Z699	Y2H Array	32814053
Intra	PRIM2	P49643	PRIM1	Homo sapiens	P49642	Y2H Prey Pooling	32296183
Intra	PRIM2	P49643	PRIM1	Homo sapiens	P49642	Y2H Array	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Diseases

Diseases	Alias
Immunodeficiency 54	Natural Killer Cell Deficiency, Familial Isolated Primary Immunodeficiency With Natural-Killer Cell Deficiency And Adrenal Insufficiency IMD54 Nkcd Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect Nkgcd Familial Isolated Natural Killer Cell Deficiency Primary Immunodeficiency Due To Mcm4 Deficiency

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (2)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-E71047	DNA Polymerase Alpha, Human		/	Unkown
HY-RS11097	PRIM2 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	PRIM2	RGD	RGD:631433
Canis familiaris	PRIM2	VGNC	VGNC:49102
Mus musculus	PRIM2	MGD	MGI:97758
Bos taurus	PRIM2	VGNC	VGNC:49563
Macaca mulatta	PRIM2	VGNC	VGNC:76398
Felis catus	PRIM2	VGNC	VGNC:69044
Others	PRIM2	NCBI