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  3. Fluorescent Dye
  4. Giemsa stain

Giemsa stain is a composite dye composed of methylene azure, methylene blue, eosin and other components. Giemsa stain stains chromatin, nuclear membranes, specific cytoplasmic components and microorganisms. Giemsa stain is used in research on cytological and parasitological staining.

For research use only. We do not sell to patients.

Giemsa stain

Giemsa stain Chemical Structure

CAS No. : 51811-82-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 g In-stock
5 g In-stock
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50 g   Get quote  

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Based on 2 publication(s) in Google Scholar

Giemsa stain Related Antibodies

Top Publications Citing Use of Products

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  • Purity & Documentation

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Description

Giemsa stain is a composite dye composed of methylene azure, methylene blue, eosin and other components. Giemsa stain stains chromatin, nuclear membranes, specific cytoplasmic components and microorganisms. Giemsa stain is used in research on cytological and parasitological staining[1][2][3].

In Vitro

Guidelines (the following is our recommended experimental protocol. This protocol is provided as a reference only, and specific procedures should be adjusted according to your actual needs).
Giemsa stain staining protocol[4]:
1. Preparation of Giemsa stain stock solution
Dissolve 1 g of Giemsa stain powder in 66 mg glycerol and heat in a 56℃ water bath for 90-120 minutes. Subsequently, add 66 mL of anhydrous methanol and mix thoroughly. Seal the container and allow it to stand at room temperature. The solution should also be filtered before use.
2. Preparation of Giemsa stain working solution
Dilution ratio 1:10-20: Giemsa stock solution: 5-10 mL, Sorensen buffer (pH 6.8): 95-100 mL
3. Staining of blood smears
a. Fix the specimen slide in anhydrous methanol for no less than 30 seconds.
b. Tilt the slide or remove it from the fixation jar to eliminate residual methanol.
c. Prepare freshly made Staining Solution No. 1 (May-Grunwald working solution, diluted with an equal volume of buffer). Place the slide flat and immerse it in the staining solution, or place it in a staining jar for 5 minutes.
d. Without washing, directly transfer the slide into freshly prepared Staining Solution No. 2 (Giemsa stain working solution, diluted with 9 volumes of buffer). Alternatively, allow the staining solution to drain from the vertically held slide before immersion. Stain for 10-15 minutes.
e. Discard the staining solution and rinse the slide once in a buffer bath.
f. Wash the slide thoroughly with running water.
g. Immerse the slide in a clean water bath for 2-5 minutes.
h. Place the slide at an angle and allow it to air dry naturally. Do not blot dry with absorbent paper.
i. Mount with a coverslip if required.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Giemsa stain Related Antibodies
Clinical Trial
Molecular Weight

291.80

Formula

C14H14ClN3S
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

C/[N+](C)=C1C=CC2=NC3=C(SC2=C/1)C=C(N)C=C3.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 20.83 mg/mL (71.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4270 mL 17.1350 mL 34.2701 mL
5 mM 0.6854 mL 3.4270 mL 6.8540 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

SDS (252 KB)

COA (246 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4270 mL 17.1350 mL 34.2700 mL 85.6751 mL
5 mM 0.6854 mL 3.4270 mL 6.8540 mL 17.1350 mL
10 mM 0.3427 mL 1.7135 mL 3.4270 mL 8.5675 mL
15 mM 0.2285 mL 1.1423 mL 2.2847 mL 5.7117 mL
20 mM 0.1714 mL 0.8568 mL 1.7135 mL 4.2838 mL
25 mM 0.1371 mL 0.6854 mL 1.3708 mL 3.4270 mL
30 mM 0.1142 mL 0.5712 mL 1.1423 mL 2.8558 mL
40 mM 0.0857 mL 0.4284 mL 0.8568 mL 2.1419 mL
50 mM 0.0685 mL 0.3427 mL 0.6854 mL 1.7135 mL
60 mM 0.0571 mL 0.2856 mL 0.5712 mL 1.4279 mL
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Giemsa stain Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Giemsa stain51811-82-6Fluorescent Dyecomposite dyecytological stainingparasitological staining
Inhibitorinhibitorinhibit

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