H-Val-Val-OH
Based on 1 Customer Validation
H-Val-Val-OH (Val-Val) is a stereochemistry-dependent substrate and inhibitor of apical oligopeptide transporters. H-Val-Val-OH undergoes apical intracellular accumulation in human intestinal cells via carrier-mediated transport, and inhibits the uptake of Cephalexin (HY-B0200) through interaction with transporters. Substitution of L-valine with D-valine in H-Val-Val-OH abolishes its binding ability to apical oligopeptide transporters.
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- Reinheit: 98.89%
- CAS. Nr.: 3918-94-3
- Formel: C10H20N2O3
- Molecular Weight:216.28
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDCK | EC50 |
0.07 mM
Compound: 80
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Activation of human PEPT1 expressed in MDCK cells
Activation of human PEPT1 expressed in MDCK cells
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[PMID: 16759105] |
| MDCK | IC50 |
0.21 mM
Compound: 80
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Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
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[PMID: 16759105] |
Val-Val (10 mM; 1 mM; 2 h) binding affinity stereoisomers for the apical oligopeptide transporter in human intestinal Caco-2 cells is linearly correlated with the distance between the N-terminal amino group and C-terminal carboxyl group (d(N1-C7)), with L-Val-L-Val showing the highest inhibitory activity (92% inhibition of Cephalexin (HY-B0200) uptake) at a d(N1-C7) distance of 5.9 Å[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 3918-94-3
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Appearance Solid
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Molecular Weight 216.28
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Formel C10H20N2O3
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Color White to off-white
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Synonyms
Val-Val
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Sequence
Val-Val
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Sequence Shortening
VV
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
H2O : 25 mg/mL (115.59 mM; Need ultrasonic)
DMSO : 9.52 mg/mL (44.02 mM; ultrasonic and adjust pH to 4 with 1M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.95 mg/mL (4.39 mM); Clear solution
This protocol yields a clear solution of ≥ 0.95 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (9.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.95 mg/mL (4.39 mM); Clear solution
This protocol yields a clear solution of ≥ 0.95 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (9.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Li J, et al. Structure-affinity relationships of Val-Val and Val-Val-Val stereoisomers with the apical oligopeptide transporter in human intestinal Caco-2 cells. J Drug Target. 1998;5(5):317-327. [Content Brief]
[2]. Islas-Preciado D, et al. Sex and BDNF Val66Met polymorphism matter for exercise-induced increase in neurogenesis and cognition in middle-aged mice. Horm Behav. 2023;148:105297. [Content Brief]
[3]. Risbrough V, et al. Generation and characterization of humanized mice carrying COMT158 Met/Val alleles. Neuropsychopharmacology. 2014;39(8):1823-1832. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 4.6236 mL | 23.1182 mL | 46.2364 mL | 115.5909 mL |
| 5 mM | 0.9247 mL | 4.6236 mL | 9.2473 mL | 23.1182 mL | |
| 10 mM | 0.4624 mL | 2.3118 mL | 4.6236 mL | 11.5591 mL | |
| 15 mM | 0.3082 mL | 1.5412 mL | 3.0824 mL | 7.7061 mL | |
| 20 mM | 0.2312 mL | 1.1559 mL | 2.3118 mL | 5.7795 mL | |
| 25 mM | 0.1849 mL | 0.9247 mL | 1.8495 mL | 4.6236 mL | |
| 30 mM | 0.1541 mL | 0.7706 mL | 1.5412 mL | 3.8530 mL | |
| 40 mM | 0.1156 mL | 0.5780 mL | 1.1559 mL | 2.8898 mL | |
| H2O | 50 mM | 0.0925 mL | 0.4624 mL | 0.9247 mL | 2.3118 mL |
| 60 mM | 0.0771 mL | 0.3853 mL | 0.7706 mL | 1.9265 mL | |
| 80 mM | 0.0578 mL | 0.2890 mL | 0.5780 mL | 1.4449 mL | |
| 100 mM | 0.0462 mL | 0.2312 mL | 0.4624 mL | 1.1559 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
H-Val-Val-OH Related Classifications
HY-126584 Related Classifications
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