HIF-1α-IN-5
Based on 1 Customer Validation
HIF-1α-IN-5 is a HIF-1α inhibitor with an IC50 value of 24 nM (in HEK293T cell). HIF-1α-IN-5 also inhibits MAO-A activity. HIF-1α-IN-5 downregulates VEGF and PDK1 mRNA expressions under hypoxia. HIF-1α-IN-5 can be used in the research of cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.95%
- CAS 番号: 2826221-10-5
- 分子式: C16H15N3O2
- 分子量:281.31
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
HIF-1α, MAO-A[1]
体外実験
HIF-1α-IN-5 (compound 15, 24 h) shows inhibitory effects against HIF-1α transcription in HEK293T cells, with an IC50 value of 24 nM[1].
HIF-1α-IN-5 (0-50 μM, 24 h) downregulates VEGF and PDK1 mRNA expressions under hypoxia in HEK293T cells[1].
HIF-1α-IN-5 (10 μM) inhibits MAO-A activity activity by 80.64%, and ADORA2A by 43.95%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HEK293T cells under hypoxia
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Concentration:0-50 μM approximately
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Incubation Time:24 h
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Result:Dose-dependently reduces VEGF and PDK1 mRNA expression.
化学情報
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CAS 番号 2826221-10-5
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性状 Solid
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分子量 281.31
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分子式 C16H15N3O2
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Color Light yellow to yellow
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SMILES
O=C(C1=NOC2=CC=CC=C12)NC3=CC=C(N(C)C)C=C3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 125 mg/mL (444.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
純度とドキュメンテーション
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データシート (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5548 mL | 17.7740 mL | 35.5480 mL | 88.8699 mL |
| 5 mM | 0.7110 mL | 3.5548 mL | 7.1096 mL | 17.7740 mL | |
| 10 mM | 0.3555 mL | 1.7774 mL | 3.5548 mL | 8.8870 mL | |
| 15 mM | 0.2370 mL | 1.1849 mL | 2.3699 mL | 5.9247 mL | |
| 20 mM | 0.1777 mL | 0.8887 mL | 1.7774 mL | 4.4435 mL | |
| 25 mM | 0.1422 mL | 0.7110 mL | 1.4219 mL | 3.5548 mL | |
| 30 mM | 0.1185 mL | 0.5925 mL | 1.1849 mL | 2.9623 mL | |
| 40 mM | 0.0889 mL | 0.4443 mL | 0.8887 mL | 2.2217 mL | |
| 50 mM | 0.0711 mL | 0.3555 mL | 0.7110 mL | 1.7774 mL | |
| 60 mM | 0.0592 mL | 0.2962 mL | 0.5925 mL | 1.4812 mL | |
| 80 mM | 0.0444 mL | 0.2222 mL | 0.4443 mL | 1.1109 mL | |
| 100 mM | 0.0355 mL | 0.1777 mL | 0.3555 mL | 0.8887 mL |
HIF-1α-IN-5 関連分類
HY-151466 関連分類
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