HIF-2α agonist 2
Based on 1 publication(s) in Google Scholar
HIF-2α agonist 2 (compound 10) is a HIF-2α agonist with an EC50 value of 1.68 μM at the dose of 20 μM. HIF-2α agonist 2 is non-cytotoxic against 786-O-HRE-Luc cells. HIF-2α agonist 2 can be used for oxygen metabolism research.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.59%
- CAS. Nr.: 2750141-15-0
- Formel: C13H8Br2N2O2S
- Molecular Weight:416.09
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) HIF-2α agonist 2
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Biologische Aktivität
Beschreibung
In Vitro
HIF-2α agonist 2 (0.001-100 μM; 24 h) dose-dependently activates HIF-2 with an EC50 value of 1.68 μM at the dose of 20 μM[1]. HIF-2α agonist 2 (0.003-20 μM; 24 h) shows no cytotoxicity against 786-O-HRE-Luc cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:786-O-HRE-Luc cell line
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Concentration:0.003-20 μM
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Incubation Time:24 hours
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Result:Showed no cytotoxicity to 786-O-Luc cells.
Chemical Information
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CAS. Nr. 2750141-15-0
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Appearance Solid
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Molecular Weight 416.09
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Formel C13H8Br2N2O2S
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Color White to off-white
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SMILES
O=S1(N=C(C2=C1C=CC=C2)NC3=CC(Br)=CC(Br)=C3)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Placenta
HIF-2α / HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia. [Abstract]2025 Dec 15:174:126-137. PMID: 41422655
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (240.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4033 mL | 12.0166 mL | 24.0333 mL | 60.0832 mL |
| 5 mM | 0.4807 mL | 2.4033 mL | 4.8067 mL | 12.0166 mL | |
| 10 mM | 0.2403 mL | 1.2017 mL | 2.4033 mL | 6.0083 mL | |
| 15 mM | 0.1602 mL | 0.8011 mL | 1.6022 mL | 4.0055 mL | |
| 20 mM | 0.1202 mL | 0.6008 mL | 1.2017 mL | 3.0042 mL | |
| 25 mM | 0.0961 mL | 0.4807 mL | 0.9613 mL | 2.4033 mL | |
| 30 mM | 0.0801 mL | 0.4006 mL | 0.8011 mL | 2.0028 mL | |
| 40 mM | 0.0601 mL | 0.3004 mL | 0.6008 mL | 1.5021 mL | |
| 50 mM | 0.0481 mL | 0.2403 mL | 0.4807 mL | 1.2017 mL | |
| 60 mM | 0.0401 mL | 0.2003 mL | 0.4006 mL | 1.0014 mL | |
| 80 mM | 0.0300 mL | 0.1502 mL | 0.3004 mL | 0.7510 mL | |
| 100 mM | 0.0240 mL | 0.1202 mL | 0.2403 mL | 0.6008 mL |
HIF-2α agonist 2 Related Classifications
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