Histone H4 (2-21)
Based on 1 Customer Validation
Histone H4 (2-21) is a substrate peptide derived from the N-terminal region of histone H4, which serves as an acyl acceptor for lysine acetyltransferases of the MYST family (KAT). Histone H4 (2-21) participates in enzymatic reactions together with acyl-coenzyme A (acyl-CoA, and undergoes lysine acetylation and propionylation modifications catalyzed by KATs such as MOF. The apparent Km of Histone H4 (2-21) for MOF is 788.8 μM, while in the picNuA4 catalytic system, the Km and Kd values of the H4 peptide are 192 μM and 251 μM, respectively. Histone H4 (2-21) can be used in studies related to histone post-translational modifications, epigenetic regulation, and lysine acylation.
For research use only. We do not sell to patients.
- Purity: 99.22%
- CAS No.: 667899-73-2
- Formula: C82H150N36O22
- Molecular Weight:1992.30
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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MOF 788.8 μM (Km) |
Histone H4 (2-21) (200 μM; 30°C; 5 min) can be acetylated by MOF in the presence of 10 μM [14C]acetyl-CoA, with an apparent Km of 788.8 μM and an apparent kcat of 37.75 min-1 for this H4 peptide[1].
Histone H4 (2-21) (100 μM; MOF: 1 μM; acyl-CoA: 200 μM; 30°C; 1 h) can be acetylated and propionylated by MOF. The acetylation sites include Lys-8, Lys-12 and Lys-16, while the propionylation sites include Lys-8 and Lys-16[2].
Histone H4 (2-21) can be acetylated by picNuA4 via an ordered sequential mechanism, with a Km of 192 μM and a Kd of 251 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 667899-73-2
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Appearance Solid
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Molecular Weight 1992.30
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Formula C82H150N36O22
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Color White to off-white
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Sequence
Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys
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Sequence Shortening
SGRGKGGKGLGKGGAKRHRK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 100 mg/mL (50.19 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (50.19 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang C, et al. Autoacetylation of the MYST Lysine Acetyltransferase MOF Protein. J Biol Chem. 2012;287(42):34917-34926. [Content Brief]
[2]. Han Z, et al. Revealing the protein propionylation activity of the histone acetyltransferase MOF (males absent on the first). The Journal of biological chemistry. 2018 Mar 02;293(9):3410-3420. [Content Brief]
[3]. Berndsen CE, et al. Catalytic mechanism of a MYST family histone acetyltransferase. Biochemistry. 2007 Jan 23;46(3):623-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5019 mL | 2.5097 mL | 5.0193 mL | 12.5483 mL |
| 5 mM | 0.1004 mL | 0.5019 mL | 1.0039 mL | 2.5097 mL | |
| 10 mM | 0.0502 mL | 0.2510 mL | 0.5019 mL | 1.2548 mL | |
| 15 mM | 0.0335 mL | 0.1673 mL | 0.3346 mL | 0.8366 mL | |
| 20 mM | 0.0251 mL | 0.1255 mL | 0.2510 mL | 0.6274 mL | |
| 25 mM | 0.0201 mL | 0.1004 mL | 0.2008 mL | 0.5019 mL | |
| 30 mM | 0.0167 mL | 0.0837 mL | 0.1673 mL | 0.4183 mL | |
| 40 mM | 0.0125 mL | 0.0627 mL | 0.1255 mL | 0.3137 mL | |
| 50 mM | 0.0100 mL | 0.0502 mL | 0.1004 mL | 0.2510 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Histone H4 (2-21) Related Classifications
HY-P1958 Related Classifications
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