HMBPP lithium  (Synonyms: (E)-4-Hydroxy-3-methyl-but-2-enyl diphosphate lithium)

HMBPP lithium is an intermediate in isoprenoid biosynthesis that can be produced by bacteria and protozoa that use the non-mevalonate pathway for isoprenoid biosynthesis. HMBPP lithium is a ligand of the internal domain of BTN3A1 and is an activator for human Vγ9/Vδ2 T cells. HMBPP lithium exhibits phagostimulant property in mosquitos^[1][2][3][4].

HMBPP (10 μM) lithium stimulates the mosquito feeding behaviour in main mosquito vectors (HMBPP-supplemented red blood cells)^[2].
HMBPP (10 μM, 12 h) lithium activates human purified γδ T cell, and increases CD69, intracellular perforin, IFN-γ, and TNF-α production when γδ T cell are incubated with GBM cells (U251, U373 and primary GBM cultures)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HMBPP (50 mg/kg, i.m.) lithium plus IL-2 (s.c., 1 million unit, once daily for 5 days) induces pro-longed accumulation of Vγ2Vδ2 T effector cells in lungs of cynomolgus monkeys During inhalational Y.pestis infection^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

279.89

C₅H₉Li₃O₈P₂

396726-03-7

Solid

White to off-white

O=P(OP(O[Li])(O[Li])=O)(O[Li])OC/C=C(C)/CO

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Hintz M, et al., Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli. FEBS Lett. 2001 Dec 7;509(2):317-22.  [Content Brief]

  [2]. Stromsky VE, et al., Plasmodium metabolite HMBPP stimulates feeding of main mosquito vectors on blood and artificial toxic sources. Commun Biol. 2021 Oct 7;4(1):1161.  [Content Brief]

  [3]. Rosso DA, et al. Glioblastoma cells potentiate the induction of the Th1-like profile in phosphoantigen-stimulated γδ T lymphocytes. J Neurooncol. 2021 Jul;153(3):403-415.  [Content Brief]

  [4]. Nguyen K,et al. The butyrophilin 3A1 intracellular domain undergoes a conformational change involving the juxtamembrane region. FASEB J. 2017 Nov;31(11):4697-4706.  [Content Brief]

  [5]. Huang D, et al. Antigen-specific Vgamma2Vdelta2 T effector cells confer homeostatic protection against pneumonic plaque lesions. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7553-8.  [Content Brief]