Hoechst 33258 analog 6

Guidelines (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
Preparation of Hoechst working solution
1.1 Preparation of stock solution
Dissolve 10 mg in 5 mL DMSO.
Note: It is recommended to store the stock solution at 4°C or -20°C, protected from light, and avoid repeated freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/mL.
Note: Please adjust the concentration of the Hoechst working solution according to actual conditions.
Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes, then discard the supernatant. Wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×10⁶/mL.
b. Add 1 mL working solution and incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and discard the supernatant.
d. Wash twice with PBS for 5 minutes each time.
e. Resuspend the cells in serum-free cell culture medium or PBS. Observe using a fluorescence microscope or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslips from the culture medium and aspirate excess medium.
c. Add 100 μL working solution, gently shake to fully cover the cells, and incubate at room temperature for 3-10 minutes.
d. Wash twice with culture medium for 5 minutes each time. Observe using a fluorescence microscope or flow cytometer.
Notes
1. Please adjust the concentration of the Hoechst working solution according to actual conditions.
2. This product is for R&D use only and is not intended for use in drugs, household, or other purposes.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.