2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor
  3. ROR
  4. huXBR1-402

huXBR1-402  (Synonyms: NBE-002 Antibody)

Cat. No.: HY-P992376
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huXBR1-402 (NBE-002 Antibody) is a high-affinity (Kd=5.8 nM) humanized chimeric rabbit/human monoclonal antibody that targets ROR1. huXBR1-402 specifically binds to the Ig/Fz domain epitope of human ROR1, directly inhibits the proliferation of ROR1-positive tumor cells and induces apoptosis of leukemia cells (apoptosis).

For research use only. We do not sell to patients.

huXBR1-402

huXBR1-402 Chemical Structure

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huXBR1-402 Related Antibodies

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Description

huXBR1-402 (NBE-002 Antibody) is a high-affinity (Kd=5.8 nM) humanized chimeric rabbit/human monoclonal antibody that targets ROR1. huXBR1-402 specifically binds to the Ig/Fz domain epitope of human ROR1, directly inhibits the proliferation of ROR1-positive tumor cells and induces apoptosis of leukemia cells (apoptosis)[1][2][3][4].

Species Reactivity

Human
In Vitro

The antibody-drug conjugate huXBR1-402-G5-PNU inhibits proliferation of ROR1-positive mantle cell lymphoma cells[1].
huXBR1-402 binds to the Ig/Fz domains of human ROR1 with affinity comparable to the parent chimeric antibody XBR1-402[2].
Fully humanized huXBR1-402 specifically binds to human ROR1 expressed on ROR1+ MCL cell lines (JeKo-1, Mino), primary CLL cells[3].
HuXBR1-402 and its antibody-drug conjugate form huXBR1-402-G5-PNU mediate Fc-dependent ADCC and ADCP activity against non-proliferative primary CLL cells, but neither mediates CDC[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

huXBR1-402 Related Antibodies
In Vivo

huXBR1-402-G5-PNU (1 mg/kg; i.v.; 2 doses on days 7 and 14 post-engraftment) significantly prolongs survival in NOD-SCID mice engrafted with human ROR1+ 697 ALL cells[3].
huXBR1-402-G5-PNU (1 mg/kg; i.p.; 3 doses on days 1, 3, and 5 post-randomization; 1 week) increases survival by 3 weeks, stabilizes disease, reduces proliferative leukemic cells, and decreases splenic and peripheral tumor burden in immune competent huROR1-TCL1 CLL mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD-SCID (6-8 week old female, immunodeficient, engrafted with human ROR1+ 697 pre-B cell ALL cells)[3]
Dosage: 1 mg/kg
Administration: i.v.; 2 doses (days 7 and 14 post-engraftment)
Result: Prolonged survival significantly compared to isotype control trastuzumab-G5-PNU.
Animal Model: C57BL/6 (8 week old, immune competent, engrafted with huROR1-TCL1 spleen-derived leukemic cells)[3]
Dosage: 1 mg/kg
Administration: i.p.; 3 doses (days 1, 3, and 5 post-randomization); 1 week
Result: Increased survival by 3 weeks compared to controls.
Stabilized disease for 3 weeks after the last dose.
Decreased numbers of EdU+ proliferating CD19+/CD5+ leukemic cells in spleens.
Significantly reduced splenic mass.
Almost halved peripheral blood tumor cell counts 4 days before euthanasia compared to vehicle and trastuzumab-G5-PNU controls.
Gene ID

4919  [NCBI]

Accession

Q01973
Target

ROR1
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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huXBR1-402 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

huXBR1-402NBE-002 AntibodyRORRAR-related orphan receptorhuROR1-TCL1 CLL miceleukemiaB-cell lymphomaMinoIg/Fz domainsprimary CLL cellsmantle cell lymphomaNOD-SCID micehuman ROR1JeKo-1Inhibitorinhibitorinhibit

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