ICAAc 

ICAAc is a solvatochromic fluorophore with reduced basicity. ICAAc demonstrates significant solvatochromic behavior across solvents of varying polarity, with a large dipole moment difference and low quantum yield in water, making it a tunable solvatochromic fluorophore. ICAAc enables pH sensing via UV-vis/fluorescence detection and in microenvironments including sodium lauryl sulfate micelle Stern layers. ICAAc acts as a supravital cell stain for epifluorescence imaging of live cancer cells^[1].

ICAAc serves as a stable and accurate physiological pH probe in aqueous buffer solutions, with a pK_a value of 7.05, enabling precise pH detection within the physiological range of pH 6-8^[1].
ICAAC (0.3 μg/mL; incubation for imaging) serves as a suitable in vitro live-cell stain for routine epifluorescence imaging of live HeLa cells, with a recommended working concentration of 0.3 μg/mL^[1].
As the polarity of the solvent increases, the emission wavelength of ICAAc increases accordingly. In water, dioxane and n-hexane, its excitation/emission maxima are 466/553 nm, 431/515 nm and 418/503 nm, respectively. The absorption maximum of ICAAc decreases with increasing pH^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

219.24

C₁₄H₉N₃

NC1=CC2=C(C=C3C(C=C([N+]#[C-])C=C3)=N2)C=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nagy M, et al. Amino-isocyanoacridines: Novel, Tunable Solvatochromic Fluorophores as Physiological pH Probes. Sci Rep. 2019;9(1):8250. Published 2019 Jun 3.