Icafolin-methyl
Based on 1 Customer Validation
Icafolin-methyl is a herbicide and plant β-tubulin (Microtubule/Tubulin) inhibitor. Icafolin-methyl binds to the β-tubulin region of the Colchicine (HY-16569) binding site, thereby blocking the polymerization of plant tubulin. As a non-selective herbicide, Icafolin-methyl exhibits post-emergence activity against weeds in both cool-season and warm-season cropping systems, including resistant ryegrass and darnel biotypes. Icafolin-methyl can be used for herbicide research.
For research use only. We do not sell to patients.
- Purity: 98.80%
- CAS No.: 2254752-21-9
- Formula: C18H18F2N2O5
- Molecular Weight:380.34
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Icafolin-methyl (10 nM; 2 weeks) strongly inhibits Arabidopsis thaliana Col-0 seedling growth, inducing morphological abnormalities consistent with mitotic disruption[1].
Icafolin-methyl (100 nM; 3 days) inhibits tobacco BY-2 cell replication, induces isodiametric cell swelling, and disrupts chromatin structure, indicating mitotic disruption via tubulin polymerization inhibition[1].
Icafolin-methyl (1.6 μM; 3 days) inhibits Chlamydomonas reinhardtii CC1690 mt+ cell growth with an EC50 of 1.3 ± 0.1 μM, preventing cell division and inducing massive cell swelling consistent with tubulin polymerization inhibition[1].
Icafolin-methyl (10 μM; throughout tubulin polymerization reaction) potently inhibits the in vitro polymerization of purified tobacco BY-2 tubulin, confirming its mode of action as a tubulin polymerization inhibitor[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Tobacco BY-2 suspension cell
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Concentration:100 nM
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Incubation Time:3 days
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Result:Stopped cell replication.
Induced isodiametric cell swelling.
Caused fragmented, dispersed DAPI-stained chromatin, consistent with tubulin polymerization inhibitor activity.
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Cell Line:Chlamydomonas reinhardtii CC1690 mt+ cell
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Concentration:1.6 μM; tested for EC50 determination
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Incubation Time:3 days
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Result:Inhibited cell growth with an EC50 of 1.3 μM.
Prevented cell division.
Induced extreme cell swelling (up to ~300-fold larger volume than untreated cells).
Chemical Information
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CAS No. 2254752-21-9
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Appearance Solid
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Molecular Weight 380.34
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Formula C18H18F2N2O5
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Color White to off-white
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SMILES
COC([C@H](OC1)C[C@H]1NC([C@]2(CC(C3=CC(F)=CC(F)=C3)=NO2)C=C)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (262.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (13.15 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6292 mL | 13.1461 mL | 26.2923 mL | 65.7307 mL |
| 5 mM | 0.5258 mL | 2.6292 mL | 5.2585 mL | 13.1461 mL | |
| 10 mM | 0.2629 mL | 1.3146 mL | 2.6292 mL | 6.5731 mL | |
| 15 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL | 4.3820 mL | |
| 20 mM | 0.1315 mL | 0.6573 mL | 1.3146 mL | 3.2865 mL | |
| 25 mM | 0.1052 mL | 0.5258 mL | 1.0517 mL | 2.6292 mL | |
| 30 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1910 mL | |
| 40 mM | 0.0657 mL | 0.3287 mL | 0.6573 mL | 1.6433 mL | |
| 50 mM | 0.0526 mL | 0.2629 mL | 0.5258 mL | 1.3146 mL | |
| 60 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0955 mL | |
| 80 mM | 0.0329 mL | 0.1643 mL | 0.3287 mL | 0.8216 mL | |
| 100 mM | 0.0263 mL | 0.1315 mL | 0.2629 mL | 0.6573 mL |
Icafolin-methyl Related Classifications
HY-170505 Related Classifications
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