Cat. No.: HY-D0720

Lucigenin

(Synonyms: NSC-151912; L-6868)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O^2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm.

For research use only. We do not sell to patients.

Purity: 99.01%
CAS No.: 2315-97-1
Formula: C₂₈H₂₂N₄O₆
Molecular Weight:510.50

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lucigenin Chemical Structure

CAS No.: 2315-97-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
- Dyeing Example
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Preparation of Lucigenin working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of Lucigenin in 0.1959 mL of DMSO to obtain 10 mM of Lucigenin.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lucigenin working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.

Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

505

Excitation (Ex)

455

Dyeing Example

Description: Lucigenin is a chemiluminescent probe, it can be used to detect superoxide anion radicals in cells under alkaline condition with green fluorescence.
Method: For HCO₃⁻ transport assay.
1. Conidia and hyphae from the strains are treated at 28°C with 0.4 M mannitol in a solution of 50 mM NaHCO₃ for 2 h.
2. Wash cells and add 0.4 M mannitol to continue recovery (2 h; 28°C). A solution of only 0.4 M mannitol served as the control.
3. Incubate strains with Lucigenin (10 mM) and observed with a fluorescence microscope.

Chemical Information

CAS No. 2315-97-1
Appearance Solid
Molecular Weight 510.50
Formula C₂₈H₂₂N₄O₆
Color Yellow to orange
SMILES

C[N+]1=C2C=CC=CC2=C(C3=C4C=CC=CC4=[N+](C)C5=C3C=CC=C5)C6=C1C=CC=C6.O=N([O-])=O.O=N([O-])=O
Synonyms

NSC-151912; L-6868
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (8)

All Cell Imaging/Staining

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Journal Impact Factor

Redox Biol

Activation of nuclear receptor pregnane-X-receptor protects against abdominal aortic aneurysm by inhibiting oxidative stress. [Abstract]

2024 Nov:77:103397. PMID: 39427444

Lucigenin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Nov:77:103397. [Abstract]

Lucigenin (10 μM, at room temperature for 15 min). The level of ROS production of HASMCs in indicated groups (n = 5 independent experiments).
Pharmacol Res

Histone deacetylase 9 exacerbates podocyte injury in hyperhomocysteinemia through epigenetic repression of Klotho. [Abstract]

2023 Dec:198:107009. PMID: 37995896
Cell Commun Signal

Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1. [Abstract]

2025 Jan 13;23(1):21. PMID: 39800699
J Transl Med

Mitochondrial ROS driven by NOX4 upregulation promotes hepatocellular carcinoma cell survival after incomplete radiofrequency ablation by inducing of mitophagy via Nrf2/PINK1. [Abstract]

2023 Mar 25;21(1):218. PMID: 36964576

Lucigenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Mar 25;21(1):218. [Abstract]

Lucigenin(100 μM). Superoxide production by NADPH oxidase was measured in total cell homogenates.
Int J Mol Med

Vaspin protects against LPS‑induced ARDS by inhibiting inflammation, apoptosis and reactive oxygen species generation in pulmonary endothelial cells via the Akt/GSK‑3β pathway. [Abstract]

2017 Dec;40(6):1803-1817. PMID: 29039444

Lucigenin purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2017 Dec;40(6):1803-1817. [Abstract]

Lucigenin(10 µM). Quantitative analysis of NADPH oxidase activity in HPMECs, as determined by lucigenin assay.
Free Radic Biol Med

TRIM16 attenuates TDP43-mediated oxidative injury by coordinating Nrf2 activation and TFR1 autophagic degradation. [Abstract]

2026 Aug 16:252:132-149. PMID: 42092406
Mar Drugs

Spermine Promotes the Formation of Conchosporangia in Pyropia haitanensis Through Superoxide Anions. [Abstract]

2025 Jul 30;23(8):309. PMID: 40863626

Lucigenin purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2025 Jul 30;23(8):309. [Abstract]

Micrographs of conchocelis stained with lucigenin. Scale bars: 50 μm. Conchocelis was treated with SPM along with NOX inhibitor DPI for 8 h and then stained with Lucigenin (0.1 μM) for 30 min.
J Fungi (Basel)

The Bicarbonate Transporter (MoAE4) Localized on Both Cytomembrane and Tonoplast Promotes Pathogenesis in Magnaporthe oryzae. [Abstract]

2021 Nov 11;7(11):955. PMID: 34829242

Lucigenin purchased from MedChemExpress. Usage Cited in: J Fungi (Basel). 2021 Nov 11;7(11):955. [Abstract]

Lucigenin (10 mM). The green fluorescence signals in hyphae. The light (left), fluorescence confocal (middle), and merge (right) microscope observations of strains hypha. Bar = 10 µm.

Solvent & Solubility

In Vitro:

DMSO : 57.14 mg/mL (111.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9589 mL

9.7943 mL

19.5886 mL

5 mM

0.3918 mL

1.9589 mL

3.9177 mL

10 mM

0.1959 mL

0.9794 mL

1.9589 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (608 KB)
- English - EN (608 KB)
- Français - FR (608 KB)
- Deutsch - DE (608 KB)
- Norwegian - NO (608 KB)
- Español - ES (608 KB)
- Swedish - SV (608 KB)
- Italian - IT (608 KB)
- Korean - KR (608 KB)
- Portuguese - PT (608 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li Y, et al. Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. J Biol Chem. 1998 Jan 23;273(4):2015-23. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9589 mL	9.7943 mL	19.5886 mL	48.9716 mL
	5 mM	0.3918 mL	1.9589 mL	3.9177 mL	9.7943 mL
	10 mM	0.1959 mL	0.9794 mL	1.9589 mL	4.8972 mL
	15 mM	0.1306 mL	0.6530 mL	1.3059 mL	3.2648 mL
	20 mM	0.0979 mL	0.4897 mL	0.9794 mL	2.4486 mL
	25 mM	0.0784 mL	0.3918 mL	0.7835 mL	1.9589 mL
	30 mM	0.0653 mL	0.3265 mL	0.6530 mL	1.6324 mL
	40 mM	0.0490 mL	0.2449 mL	0.4897 mL	1.2243 mL
	50 mM	0.0392 mL	0.1959 mL	0.3918 mL	0.9794 mL
	60 mM	0.0326 mL	0.1632 mL	0.3265 mL	0.8162 mL
	80 mM	0.0245 mL	0.1224 mL	0.2449 mL	0.6121 mL
	100 mM	0.0196 mL	0.0979 mL	0.1959 mL	0.4897 mL

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Lucigenin

Publications Citing Use of MedChemExpress (MCE) Lucigenin

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Lucigenin Related Classifications

HY-D0720 Related Classifications