Thymus factor X

The experiment is carried out using 134 mice divided into three groups: Group I: 46 mice infected with T. spiralis and treated with Thymus factor X (TFX); Group II: 42 uninfected mice treated with Thymus factor X; and Control group: 46 mice infected with T. spiralis and not treated with Thymus factor X. Thymus factor X is administered subcutaneously at a dose of 15 mg/kg. The first dose is administered on the third day before the mice are infected with T. spiralis. The dose is repeated at 24 h intervals until the thirteenth day after infection. In all, 17 doses are administered. On days 7, 14, 21, 28, 35, 42, and 60 after infection, six mice from each group are sacrificed using halothane. The spleen, mesenteric lymph nodes and masseter muscles are excised from each mouse and transferred to sterile phospho-buffered saline at 4°C^[1].

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