Indirubin-3′-oxime  (Synonyms: IDR3O; I3O)

Indirubin-3′-oxime (IDR3O), a synthetic derivative of indirubin, is a potent inhibitor of cyclin-dependent kinases (CDKs) and glycogen synthase kinase 3β (GSK3β). Indirubin-3′-oxime directly inhibits the activity of all three isoforms of JNK (JNK1, JNK2, and JNK3), with IC₅₀s of 0.8 μM, 1.4 μM, and 1.0 μM, respectively. Indirubin-3′-oxime can enhance height growth via activation of Wnt/β-catenin signaling in chondrocytes^[1][2][3].

In cerebellar granule neurons (CGNs), Indirubin-3′-oxime blocks c-Jun phosphorylation induced by potassium withdrawal and prevented CGNs from apoptosis in a dose dependent manner^[1].
Indirubin-3′-oxime (IDR3O) (PC12 cells; 10 μM) significantly prevent 6OHDA-induced decrease of nuclear localized MEF2D expression^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Indirubin-3′-oxime (0.05 or 0.5 mg/kg; i.p.; daily for 2 or 10 weeks) enhances tibial longitudinal growth in mice without adverse changes in bone thickness parameters^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

277.28

Solid

C₁₆H₁₁N₃O₂

667463-82-3

O/N=C1C2=C(C=CC=C2)NC\1=C3C4=C(C=CC=C4)NC\3=O

Room temperature in continental US; may vary elsewhere.

• [1]. Xie Y, et al. Indirubin-3'-oxime inhibits c-Jun NH2-terminal kinase: anti-apoptotic effect in cerebellar granule neurons. Neurosci Lett. 2004;367(3):355-359.  [Content Brief]

  [2]. Hu S, et al. Indirubin-3-Oxime Effectively Prevents 6OHDA-Induced Neurotoxicity in PC12 Cells via Activating MEF2D Through the Inhibition of GSK3β. J Mol Neurosci. 2015;57(4):561-570.  [Content Brief]

  [3]. Choi S, et al. Indirubin-3'-oxime stimulates chondrocyte maturation and longitudinal bone growth via activation of the Wnt/β-catenin pathway. Exp Mol Med. 2019;51(9):1-10. Published 2019 Sep 12.  [Content Brief]