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Cat. No.: HY-DY1118

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INDO 1 pentapotassium (solution)

INDO 1 pentapotassium solution is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 pentapotassium solution binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).

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CAS No.: 132319-56-3
Formule: C₃₂H₂₆K₅N₃O₁₂
Masse moléculaire:840.05

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Please store the product under the recommended conditions in the Certificate of Analysis.

INDO 1 pentapotassium (solution) Chemical Structure

CAS No.: 132319-56-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Description

In Vitro

INDO 1 pentapotassium solution enables accurate measurement of absolute cytoplasmic Ca²⁺ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster^[1].
INDO 1 pentapotassium solution binds extensively to proteins isolated from rabbit ventricular myocytes^[2].
INDO 1 pentapotassium solution is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent K_D·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 132319-56-3
Masse moléculaire 840.05
Formule C₃₂H₂₆K₅N₃O₁₂
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Hardie RC, et al. INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1996 May 01;16(9):2924-33.

[2]. Hove-Madsen L, et al. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophysical journal. 1992 Jul;63(1):89-97.

[3]. Jacquemond V, et al. Indo-1 fluorescence signals elicited by membrane depolarization in enzymatically isolated mouse skeletal muscle fibers. Biophysical journal. 1997 Aug;73(2):920-8.

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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INDO 1 pentapotassium (solution)

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INDO 1 pentapotassium (solution)

Activité biologique

Chemical Information

Pureté et documentation

Références

Calculators

Help & FAQs

INDO 1 pentapotassium (solution) Related Classifications

HY-DY1118 Related Classifications

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