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Cat. No.: HY-DY1118

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INDO 1 pentapotassium (solution)

INDO 1 pentapotassium solution is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 pentapotassium solution binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).
Solvent and concentration: ddH2O: 1 mM

For research use only. We do not sell to patients.

CAS No.: 132319-56-3
Formula: C₃₂H₂₆K₅N₃O₁₂
Molecular Weight:840.05

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

INDO 1 pentapotassium (solution) Chemical Structure

CAS No.: 132319-56-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
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Biological Activity

Description

In Vitro

INDO 1 pentapotassium solution enables accurate measurement of absolute cytoplasmic Ca²⁺ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster^[1].
INDO 1 pentapotassium solution binds extensively to proteins isolated from rabbit ventricular myocytes^[2].
INDO 1 pentapotassium solution is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent K_D·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 132319-56-3
Molecular Weight 840.05
Formula C₃₂H₂₆K₅N₃O₁₂
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hardie RC, et al. INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1996 May 01;16(9):2924-33.

[2]. Hove-Madsen L, et al. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophysical journal. 1992 Jul;63(1):89-97.

[3]. Jacquemond V, et al. Indo-1 fluorescence signals elicited by membrane depolarization in enzymatically isolated mouse skeletal muscle fibers. Biophysical journal. 1997 Aug;73(2):920-8. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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