2. Immunology/Inflammation
  3. LILRB
  4. IO-202

IO-202 is a high-affinity LILRB4/ILT3 binder and myeloid checkpoint inhibitor. IO-202 blocks APOE binding and LILRB4 activation to reverse T-cell suppression and enhance T-cell cytotoxicity, while eliminating LILRB4-high-expressing leukemic blasts via ADCC and ADCP mechanisms. IO-202 promotes dendritic cell maturation and antigen presentation, reshapes the phenotype of tumor-associated macrophages, and reduces myeloid-derived suppressor cells. IO-202 is widely applicable to research on relapsed/refractory acute myeloid leukemia (AML), chronic myelomonocytic leukemia (CMML), and solid tumors.

For research use only. We do not sell to patients.

IO-202

IO-202 Chemical Structure

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Description

IO-202 is a high-affinity LILRB4/ILT3 binder and myeloid checkpoint inhibitor. IO-202 blocks APOE binding and LILRB4 activation to reverse T-cell suppression and enhance T-cell cytotoxicity, while eliminating LILRB4-high-expressing leukemic blasts via ADCC and ADCP mechanisms. IO-202 promotes dendritic cell maturation and antigen presentation, reshapes the phenotype of tumor-associated macrophages, and reduces myeloid-derived suppressor cells. IO-202 is widely applicable to research on relapsed/refractory acute myeloid leukemia (AML), chronic myelomonocytic leukemia (CMML), and solid tumors[1][2][3].

Isotype

Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity

Human
IC50 & Target[1]

LILRB4

 

In Vitro

IO-202 reverses AML cell-mediated T-cell suppression and activates T-cell cytotoxicity against leukemic cells in vitro via high-affinity binding to human LILRB4 and blockade of APOE-mediated LILRB4 activation[1].
IO-202 (15 μg/mL; 2 days) enhances DC activation (increased CD86 and HLA-DR expression) and reduces a tolerogenic phenotype (decreased CD209 expression) when used to treat healthy human donor monocyte-derived dendritic cells during LPS stimulation[3].
IO-202 (15 μg/mL; 2 days during Mo-DC culture, 4 days during T cell co-culture) enhances DC activation marker expression, IL-12 production, and T cell-derived IFNγ production when used to treat human monocyte-derived dendritic cells during CD40L-induced maturation/activation and subsequent allogeneic T cell co-culture, but has no effect on immature Mo-DCs[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IO-202 Related Antibodies

ELISA Assay[3]

Cell Line: human monocyte-derived dendritic cells
Concentration: 15 μg/mL
Incubation Time: 2 days during Mo-DC culture, 4 days during T cell co-culture
Result: Enhanced DC activation marker expression, IL-12 production, and T cell-derived IFNγ production.
In Vivo

IO-202 (10 mg/kg; administered on day 10 and day 20 post tumor implant) combined with 10 Gy radiation therapy induces potent tumor growth inhibition and rebalances the tumor immune infiltrate toward a pro-inflammatory phenotype in a transgenic mouse Lewis lung carcinoma model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Cx3cr1-Cre/Rosa26-LILRB4 transgenic[3]
Dosage: 10 mg/kg
Administration: administered on day 10 and day 20 post tumor implant
Result: Achieved significant tumor growth inhibition with mean tumor volume at day 27 post tumor implant being substantially lower.
Reduced the frequencies of myeloid-derived suppressor cells (including PMN-MDSCs) among tumor-infiltrating leukocytes.
Increased the frequencies of conventional CD4+ T cells and NKT cells among tumor-infiltrating leukocytes.
Decreased the expression of the anti-inflammatory marker CD206 on tumor-associated macrophages (TAMs).
Increased MHCII expression on TAMs.
Gene ID

11006  [NCBI]

Accession
Target

LILRB4/ILT3/CD85k
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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IO-202 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IO-202IO202IO 202LILRBLeukocyte Immunoglobulin-Like Receptor Subfamily BLILRB4dendritic cellAPOEacute myeloid leukemiaCD8+ T cellschronic myelomonocytic leukemiatumor-associated macrophagescynomolgus monkeysmonocytic myeloid-derived suppressor cellseffector T-cellsInhibitorinhibitorinhibit

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