Isoxadifen-ethyl
Based on 1 Customer Validation
Isoxadifen-ethyl is a Herbicide safener that reduces herbicide toxicity to crops. Isoxadifen-ethyl enhances maize tolerance to Nicosulfuron (HY-B1876) by regulating detoxification gene expression; it reduces the phytotoxicity and damage induced by Cyhalofop-butyl (HY-B0861) in rice. Isoxadifen-ethyl is applicable to herbicide-related research.
For research use only. We do not sell to patients.
- Purity : 99.28%
- CAS No.: 163520-33-0
- Formula: C18H17NO3
- Molecular Weight:295.34
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
Isoxadifen-ethyl (33 mg/L; 24 h) significantly upregulates the expression levels of ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 in the leaves of maize cultivar Zhenghuangnuo 2, while downregulating the expression of ZmGSTIII and ZmGST27[1].
Isoxadifen-ethyl (33 mg/L; 24 h) significantly upregulates the expression levels of ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 in leaves of maize variety Zhengdan 958, while downregulating the expression levels of ZmGSTIII, ZmGST27 and ZmGT1[1].
Isoxadifen-ethyl (33 mg/L; 4-48 h) significantly upregulates the expression levels of ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 in the leaves of maize varieties Zhenghuangnuo 2 and Zhengdan 958[1].
Isoxadifen-ethyl acts as a broad-spectrum, multi-crop herbicide safener that protects maize and rice from phytotoxicity induced by Foramsulfuron (HY-W422402), Mesotrione (HY-12853), Isoxaflutole (HY-116139) and fenoxaprop-P-ethyl[2].
Isoxadifen-ethyl (compound 1) (50-100 μM; 5 d) effectively alleviates the phytotoxicity of Cyhalofop-butyl (HY-B0861) to cultivated rice seedlings in agar-based assays, with a plant height recovery rate of 95.86% and a fresh weight recovery rate of 89.87%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 163520-33-0
-
Appearance Solid
-
Molecular Weight 295.34
-
Formula C18H17NO3
-
Color White to light yellow
-
SMILES
O=C(C1=NOC(C2=CC=CC=C2)(C3=CC=CC=C3)C1)OCC
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (338.59 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (16.93 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (16.93 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
-
RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
-
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
-
Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (598 KB)
- English - EN (598 KB)
- Français - FR (598 KB)
- Deutsch - DE (598 KB)
- Norwegian - NO (598 KB)
- Español - ES (598 KB)
- Swedish - SV (598 KB)
- Italian - IT (598 KB)
- Korean - KR (598 KB)
- Portuguese - PT (598 KB)
-
Handling Instructions (2659 KB)
References
[1]. Sun L, et al. The expression of detoxification genes in two maize cultivars by interaction of isoxadifen-ethyl and nicosulfuron. Plant physiology and biochemistry : PPB. 2018 Aug;129:101-108. [Content Brief]
[2]. Jia L, et al. Research Progress in the Design and Synthesis of Herbicide Safeners: A Review. Journal of agricultural and food chemistry. 2022 May 11;70(18):5499-5515. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3859 mL | 16.9296 mL | 33.8593 mL | 84.6482 mL |
| 5 mM | 0.6772 mL | 3.3859 mL | 6.7719 mL | 16.9296 mL | |
| 10 mM | 0.3386 mL | 1.6930 mL | 3.3859 mL | 8.4648 mL | |
| 15 mM | 0.2257 mL | 1.1286 mL | 2.2573 mL | 5.6432 mL | |
| 20 mM | 0.1693 mL | 0.8465 mL | 1.6930 mL | 4.2324 mL | |
| 25 mM | 0.1354 mL | 0.6772 mL | 1.3544 mL | 3.3859 mL | |
| 30 mM | 0.1129 mL | 0.5643 mL | 1.1286 mL | 2.8216 mL | |
| 40 mM | 0.0846 mL | 0.4232 mL | 0.8465 mL | 2.1162 mL | |
| 50 mM | 0.0677 mL | 0.3386 mL | 0.6772 mL | 1.6930 mL | |
| 60 mM | 0.0564 mL | 0.2822 mL | 0.5643 mL | 1.4108 mL | |
| 80 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0581 mL | |
| 100 mM | 0.0339 mL | 0.1693 mL | 0.3386 mL | 0.8465 mL |
Isoxadifen-ethyl Related Classifications
HY-130361 Related Classifications
Powered by Bioz