Centaurein
Centaurein, a flavonoid, is an IFN-γ promoter enhancer. Centaurein up-regulates the activity of NFAT and NF-κB enhancers. Centaurein increases the IFN-γ expression in T and NK cells and the serum IFN-γ level in mice. Centaureidin completely relaxes the contractions in intact rat aortic rings. Centaurein effectively protects mice against Listeria infection[1][2][3][4].
For research use only. We do not sell to patients.
- CAS No.: 35595-03-0
- Formula: C24H26O13
- Molecular Weight:522.46
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Centaurein (5-100 μg/mL, 24-72 h) can stimulate IFN-γ transcription in a T cell line (Jurkat cells) and splenocytes and up-regulates the activity of NFAT and NF-κB enhancers[1][2][3].
Centaurein (100 μg/mL, 24 h) up-regulates the transcription of T-bet and IFN-γ but not GATA-3 and IL-4 in T cells[2].
Centaurein (6 h) pretreatment reduces Listeria infection in macrophages from 46% to 15%, an effect partially reversed (34% infection) by IFN-γ-neutralizing antibody[2].
Centaureidin (10-100 μM) completely relaxes the contractions induced by Norepinephrine (NA) (HY-13715) (IC50 = 16.7 μM) or by a high K concentration (IC50 =16.1 μM) in intact rat aortic rings concentration-dependently[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:T cells
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Concentration:100 μg/mL
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Incubation Time:24 h
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Result:Increased the expression of T-bet by 5-fold.
Enhanced the transcription of IFN-γ but not IL-4 or GATA-3.
In Vivo
Centaurein (20 μg/mouse, i.p., single dose) augments the serum IFN-γ level in mice, which peaked 24 h post-injection[2].
Centaurein (20 μg/mouse, i.p., single dose) protects against and treats Listeriainfection in mice via up-regulation of IFN-γ and macrophage activation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Listeria infection model established in 6-8 weeks C57BL/6J mice and IFN-γ−/− C57BL/6J mice[2]
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Dosage:20 μg/mouse
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Administration:i.p. for single dose
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Result:Protected mice against Listeria infection in a dose-dependent fashion.
Did not protect IFN-γ-knockout mice against Listeria infection, but Ampicillin (HY-B0522) at 1000 μg/mL could protect against Listeria infection.
Rescued 30% of the mice infected with a lethal dose of Listeria (2 × 10 6 CFU).
Rescued 70% of the mice that received the lethal dose of Listeria in combination with antibiotics (5 μg/mouse, 2 times/day for 3 days)
Chemical Information
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CAS No. 35595-03-0
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Molecular Weight 522.46
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Formula C24H26O13
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SMILES
O=C1C2=C(O)C(OC)=C(O[C@@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)C=C2OC(C4=CC(O)=C(C=C4)OC)=C1OC
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
References
[1]. Chang SL, et al. Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression. J Ethnopharmacol. 2007 Jun 13;112(2):232-6. [Content Brief]
[2]. Chang SL, Yeh HH, Lin YS, Chiang YM, Wu TK, Yang WC. The effect of centaurein on interferon-gamma expression and Listeria infection in mice. Toxicol Appl Pharmacol. 2007 Feb 15;219(1):54-61. [Content Brief]
[3]. Nikiema WA, et al. Systematic Review of Chemical Compounds with Immunomodulatory Action Isolated from African Medicinal Plants. Molecules. 2024 Apr 26;29(9):2010. [Content Brief]
[4]. Orallo F, et al. Preliminary study of the potential vasodilator effects on rat aorta of centaurein and centaureidin, two flavonoids from Centaurea corcubionensis. Planta Med. 1998 Mar;64(2):116-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Centaurein Related Classifications
HY-N13614 Related Classifications
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