TH1217
Based on 1 publication(s) in Google Scholar
TH1217 (ZINC1775962367) is a potent and selective dCTPase pyrophosphatase 1 (dCTPase) inhibitor, with an IC50 of 47 nM. TH1217 enhances the cytotoxic effect of cytidine analogues in leukemia cells. TH1217 also could modulate SARS-Cov-2 interactors, so it shows activity of against COVID-19.
For research use only. We do not sell to patients.
- Purity : 97.04%
- CAS No.: 1862212-48-3
- Formula: C20H17BCl2N4O6
- Molecular Weight:491.09
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TH1217
More
Biological Activity
Description
IC50 & Target
IC50: 47 nM (dCTPase)[1]
In Vitro
TH1217 (compound 30) inhibits dCTPase and enhances aqueous solubility (>100 μM) and improves plasma stability in vitro(t4h=86%)[1].
TH1217 presents suitable mouse microsomal half-lives (109 minutes)[1].
TH1217 displays well cell permeability (8.66×10-6/1.30×10-3 cm/s) and CYP inhibition[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 1862212-48-3
-
Appearance Solid
-
Molecular Weight 491.09
-
Formula C20H17BCl2N4O6
-
Color White to off-white
-
SMILES
CC1=NC2=C(C(Cl)=C(Cl)C=C2N1CC3=CC=C(C=C3)[B-]45OC(C[N+]4(C)CC(O5)=O)=O)[N+]([O-])=O
-
Synonyms
ZINC1775962367
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Nucleic Acids Res
COVID19 Drug Repository: text-mining the literature in search of putative COVID19 therapeutics. [Abstract]2021 Jan 8;49(D1):D1113-D1121. PMID: 33166390
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (254.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. Llona-Minguez S, et, al. Discovery of the First Potent and Selective Inhibitors of Human dCTP Pyrophosphatase 1. Med Chem. 2016 Feb 11; 59(3): 1140-1148. [Content Brief]
[2]. Gordon DE, et, al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. bioRxiv. 2020 Mar 22; 2020.03.22.002386. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0363 mL | 10.1814 mL | 20.3629 mL | 50.9072 mL |
| 5 mM | 0.4073 mL | 2.0363 mL | 4.0726 mL | 10.1814 mL | |
| 10 mM | 0.2036 mL | 1.0181 mL | 2.0363 mL | 5.0907 mL | |
| 15 mM | 0.1358 mL | 0.6788 mL | 1.3575 mL | 3.3938 mL | |
| 20 mM | 0.1018 mL | 0.5091 mL | 1.0181 mL | 2.5454 mL | |
| 25 mM | 0.0815 mL | 0.4073 mL | 0.8145 mL | 2.0363 mL | |
| 30 mM | 0.0679 mL | 0.3394 mL | 0.6788 mL | 1.6969 mL | |
| 40 mM | 0.0509 mL | 0.2545 mL | 0.5091 mL | 1.2727 mL | |
| 50 mM | 0.0407 mL | 0.2036 mL | 0.4073 mL | 1.0181 mL | |
| 60 mM | 0.0339 mL | 0.1697 mL | 0.3394 mL | 0.8485 mL | |
| 80 mM | 0.0255 mL | 0.1273 mL | 0.2545 mL | 0.6363 mL | |
| 100 mM | 0.0204 mL | 0.1018 mL | 0.2036 mL | 0.5091 mL |
TH1217 Related Classifications
HY-135909 Related Classifications
Powered by Bioz